A Preliminary Study on the Effects of Several Frequently Used Chemical Insecticides on the Growth of Diverse Bacillus thuringiensis Strains

doi:10.16409/j.cnki.2095-039x.2021.09.010

Abstract

Abstract: Bacillus thuringiensis(Bt) can produce a variety of pesticidal active substances and is one of the most widely used microbial insecticides.Recent studies have shown that Bt is of high control of chemical insecticide resistant pests and that combined use of Bt with chemical insecticides can improve the control efficiency and reduce the usage of chemical insecticides.To improve the synergistic pest control between Bt and chemical insecticides,this study investigated the compatibility of different subspecies of Bt strains with frequenctly used chemical insecticides.Pan-genomic analysis showed that Bt strains from different subspecies differed significantly in their genetic composition,suggesting that Bt strains may respond differently to chemical insecticides.Further analysis of the effect of chemical insecticides on Bt proliferation showed that chlorfenapyr inhibited most of the tested Bt strains,indicating that the compatibility between Bt and chemical insecticides needs to be measured when they are used in combination.The Bt insecticide subspecies Bt kurstaki(Btk) and Bt aizawai (Bta) frequently used for the control of lepidopteran pests showed synergistical control with beta-cypermethrin.To Btk strain HD1,chlorantraniliprole and indoxacarb exhibited some inhibitory effect,while chlorfluazuron promoted growth,indicating that HD1 and chlorfluazuron can be used synergistically.For Bta strain G03,indoxacarb had less negative effect,chlorfluazuron was inhibitory,and chlorantraniliprole was promotional,showing that G03 and its derivatives can be used synergistically with chlorantraniliprole and indoxacarb.The results indicate that the sensitivity of Bt to chemical insecticides differ between strains,indicating that combinations between Bt and chemicals with high compatibility can be obtained through screening.Our data also shed light on the further combination of Bt insecticide and chemical insecticide,which is important for the reduction of chemical pesticide application.

Key words: Bacillus thuringiensis, chemical insecticide, multiplication effect, pesticide compatibility, pest control

CLC Number:

S476.1

SHU Changlong, ZHANG Xian, WANG Kui, CAO Beibei, ZHANG Jie. A Preliminary Study on the Effects of Several Frequently Used Chemical Insecticides on the Growth of Diverse Bacillus thuringiensis Strains[J]. Chinese Journal of Biological Control, 2022, 38(5): 1166-1173.

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