Toxicity and Field Control Efficacy of Plant Spray oil and Metarhizium anisopliae to Phyllotreta striolata

doi:10.16409/j.cnki.2095-039x.2022.03.022

Abstract

Abstract: Phyllotreta striolata(Fabricus) is an important pest of Brassicaceae that has invited long-term application of chemical pesticides,which not only enhances the resistance of P.striolata,but also leads to the increase of pesticide residues in vegetables and endangers human health.Therefore,the employment of green control measures has attracted much attention in recent years.In this study,we measured the laboratory virulence of plant spray oil emulsion,Metarhizium anisopliae and their mixture against P.striolata adults and field effectiveness of the oil-bacteria mixture.The bioassay results showed that the mortality rates of P.striolata adults ranged between 37.93%―100% on the 2^nd day post application of the plant spray oil emulsion diluted at 50―800 folds and between 32.2%―93.22% on the 5^th day post application of 1.25×10⁷―2×10⁸ spores/mL concentration of M.anisopliae,and were more than 95% on the 1^st day post application of the mixture of plant spray oil emulsion and M.anisopliae.The mixture exhibited a synergistic effect on virulence and significantly shortened the median lethal time of P.striolata adults.In the field tests,the relative efficacy and population decline rates of P.striolata adults were both more than 60% on the 6^th day post treatment with a mixture ofplant spray oil emulsion diluted at 100 folds and 1×10⁸ spores/mL of M.anisopliae and reached 89.80% and 74.62%,respectively,on the 8^th day post treatment,which were significantly higher than those obtained with the individual doses at the same time and concentration.The results of this study indicate that plant spray oil emulsion and M.anisopliae have significant synergistic effects in the control of P.striolata.This study also serves as a practical case for exploring and utilizing the synergistic effect between biogenic pesticides for pest control.

Key words: plant spray oil, Metarhizium anisopliae, Phyllotreta striolata, indoor toxicity, field control efficacy

CLC Number:

S476.2

YAN Jianquan, LIU Hao, CHEN Lijia, LU Huilin, CHEN Fenghao, HOU Junxiao, YANG Rong, OUYANG Xiongfa, OUYANG Gecheng, MENG Xiang. Toxicity and Field Control Efficacy of Plant Spray oil and Metarhizium anisopliae to Phyllotreta striolata[J]. Chinese Journal of Biological Control, 2022, 38(5): 1174-1183.

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