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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (5): 1174-1183.DOI: 10.16409/j.cnki.2095-039x.2022.03.022

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Toxicity and Field Control Efficacy of Plant Spray oil and Metarhizium anisopliae to Phyllotreta striolata

YAN Jianquan1,2, LIU Hao1, CHEN Lijia3, LU Huilin1, CHEN Fenghao1,2, HOU Junxiao4, YANG Rong4, OUYANG Xiongfa5, OUYANG Gecheng1, MENG Xiang1   

  1. 1. Guangdong Key Laboratory of Animal Conservation and Resource Utilization/Guangdong Public Laboratory of Wild Animal Conservation and Utilization/Institute of Zoology, Guangdong Academy of Science, Guangzhou 510260, China;
    2. College of Plant Protection, South China Agricultural University, Guangzhou 510642, China;
    3. College of Life Sciences, Guangzhou University, Guangzhou 510006, China;
    4. Guangzhou Jiaoyang Agriculture Company, Guangzhou 510653, China;
    5. Guangzhou Oulian Biotechnology Co., Ltd., Guangzhou 510520, China
  • Received:2021-09-11 Published:2022-10-20

Abstract: Phyllotreta striolata(Fabricus) is an important pest of Brassicaceae that has invited long-term application of chemical pesticides,which not only enhances the resistance of P.striolata,but also leads to the increase of pesticide residues in vegetables and endangers human health.Therefore,the employment of green control measures has attracted much attention in recent years.In this study,we measured the laboratory virulence of plant spray oil emulsion,Metarhizium anisopliae and their mixture against P.striolata adults and field effectiveness of the oil-bacteria mixture.The bioassay results showed that the mortality rates of P.striolata adults ranged between 37.93%―100% on the 2nd day post application of the plant spray oil emulsion diluted at 50―800 folds and between 32.2%―93.22% on the 5th day post application of 1.25×107―2×108 spores/mL concentration of M.anisopliae,and were more than 95% on the 1st day post application of the mixture of plant spray oil emulsion and M.anisopliae.The mixture exhibited a synergistic effect on virulence and significantly shortened the median lethal time of P.striolata adults.In the field tests,the relative efficacy and population decline rates of P.striolata adults were both more than 60% on the 6th day post treatment with a mixture ofplant spray oil emulsion diluted at 100 folds and 1×108 spores/mL of M.anisopliae and reached 89.80% and 74.62%,respectively,on the 8th day post treatment,which were significantly higher than those obtained with the individual doses at the same time and concentration.The results of this study indicate that plant spray oil emulsion and M.anisopliae have significant synergistic effects in the control of P.striolata.This study also serves as a practical case for exploring and utilizing the synergistic effect between biogenic pesticides for pest control.

Key words: plant spray oil, Metarhizium anisopliae, Phyllotreta striolata, indoor toxicity, field control efficacy

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