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Chinese Journal of Biological Control ›› 2023, Vol. 39 ›› Issue (5): 1172-1185.DOI: 10.16409/j.cnki.2095-039x.2023.02.052

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Biocontrol Fungus against Base Rot of Bambusa pervariabilis × Dendrocalamopsis grandis and Its Preparation and Effect of Water-Dispersing Granule

GU Xiaomin1,2, YU Jiawen1, ZHU Tianhui1, CHEN Youzhong3, LIU Han2, LI Shujiang1   

  1. 1. Key Laboratory of Forest Resources Conservation and Ecological Security in the Upper Reaches of Yangtze River, National Forestry and Grassland Administration/College of Forestry, Sichuan Agricultural University, Chengdu 611130, China;
    2. Sichuan Ganzi Institute of Forestry Science, Kangding 626001, China;
    3. Sichuan Guoguang Agrochemical Co., LTD., Chengdu 610100, China
  • Received:2023-03-07 Online:2023-10-08 Published:2023-10-25

Abstract: To develop an effective preparation for controlling the base rot disease of Bambusa pervariabilis × Dendrocalamopsis grandis, the fungi were isolated from the rhizosphere soil of healthy bamboo by the dilution plate method. The strain RS05 was screened by the plate confrontation method and identified as Trichoderma spiralis by the morphological and molecular biological analyses. Based on the optimization of basic medium and fermentation conditions, the optimum carrier and additive of water-dispersible granule were screened. The results showed that the optimum medium for RS05 was PSA. The fermentation conditions were as follows: inoculation amount 8%, pH 6, fermentation time 6 d, 180 r/min, light culture at 26 ℃~27 ℃. The best prescription of water-dispersible granules were comprised 3% Tween 20 (wetting agent), 0.3% dextrin (UV protection agent), 3% soluble starch (binding agent), 5% sodium chloride (disintegrating agent), 0.3% calcium carbonate (stabilizer), 20% spore suspension, and silica gel (carrier) complemented 100%. The results of pot experiments indicated that the control effect of RS05 water-dispersible granules was higher than 88% when diluted less than 200 times, slightly higher than 86% of commercially available chemical preparations, and significantly higher than 64% of commercially available biocontrol preparations. In conclusion, the water-dispersible granules of T. spiralis RS05 has excellent control effect on the base rot disease of B. pervariabilis×D. grandis.

Key words: Trichoderma spiralis, water-dispersible granule, fermentation, control effect, base rot

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