Preparation of Bacillus amyloliquefaciens PHODG36 Granules Based on Broccoli residues as Carrier and Its Control Effect on Cotton Verticillium Wilt

doi:10.16409/j.cnki.2095-039x.2023.02.050

Abstract

Abstract: In this study, a microbial agent for controlling cotton verticillium wilt was developed using biocontrol and growth-promoting of Bacillus amyloliquefaciens PHODG36 as the active ingredient, dried broccoli residues powder, and peat as the filler. The effects of PHODG36 granules on cotton seedling emergence and plant biomass were studied, and the control effects of the microbial agent on cotton verticillium wilt were evaluated through greenhouse pot experiments and field plot experiments. The results showed that the microbial agent had a black, short columnar appearance, an average length of 8±1 mm, and spore content was 1.5×10⁹ CFU/g. Compared with the blank control, there was no significant difference in the corrected seedling rates among treatments with concentration of 0.1% to 0.5% of the microbial agent added to the culture substrate, but with the increase of the microbial agent concentration (1% and 2%), the corrected seedling rates showed a significant downward trend. When the concentration of microbial agent was 0.3%, the growth promotion rate on plant height, fresh and dry weight of plants were 14.53%, 16.38% and 17.05%, respectively. Taking the above factors into consideration, the optimal concentration of microbial agent was 0.3%, and the control effect on cotton verticillium wilt was 61.60% in greenhouse experiment. The control effect of the agent on cotton verticillium wilt was studied at the dosage of 500, 1000 and 1500 kg/667 m²by spreading application method in field. The results showed that the maximum control effect on cotton verticillium wilt was 67.28%, when the agent was 500 kg/667 m². Comprehensive analysis showed that B. amyloliquefaciens PHODG36 granules could effectively reduce the incidence of cotton verticillium wilt, and provide a product reserve for green prevention and control this disease.

Key words: Bacillus amyloliquefaciens, dried broccoli residue powder, granules, cotton verticillium wilt, control effect

CLC Number:

S476

ZHAO Weisong, LI Shezeng, ZHAO Mingzhu, GUO Qinggang, LU Xiuyun, GOU Jianjun, MA Ping. Preparation of Bacillus amyloliquefaciens PHODG36 Granules Based on Broccoli residues as Carrier and Its Control Effect on Cotton Verticillium Wilt[J]. Chinese Journal of Biological Control, 2023, 39(5): 1186-1193.

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