Screening and Biocontrol Effect of Antagonistic Bacterium B44R-1 against Phytophthora parasitica

doi:10.16409/j.cnki.2095-039x.2022.06.012

Abstract

Abstract: To obtain excellent antagonistic bacteria of Phytophthora nicotiana in tobacco, the antagonistic strains of P. nicotiana were isolated from the rhizosphere soil and root of tobacco, and the antagonistic strains with strong inhibitory effects were screened. Afterwards, the antagonistic strains were further identified by morphological analysis, physiological and biochemical test, as well as 16S rDNA gene sequencing analysis. Besides, the effects of disease control and growth promotion on tobacco were also evaluated. The results showed that 39 antagonistic bacteria were isolated, and the strain B44R-1 with the best antagonistic effect was screened and preliminarily identified as Acinetobater pittii. The control efficacy of strain B44R-1 on tobacco black shank disease reached 55.37%. Moreover, this strain had obvious effect on the growth and development of tobacco root. Compared with the control, the agronomic traits of the treated tobacco plants were significantly improved, including fresh weight, dry weight, plant height, stem circumference, leaf number and maximum leaf area. In conclusion, the antagonistic bacterium A. pittii has significant control effect on tobacco black shank disease and has good biocontrol potential.

Key words: tobacco black shank, antagonistic microflora, Acinetobater pittii, control effect, growth promoting effect

CLC Number:

S476

LIU Tianbo, Zhao Yuqiang, TENG Kai, ZHOU Xiangping, XIAO Yansong, CAI Hailin, XIAO Zhipeng, CHEN Wu, TANG Qianjun. Screening and Biocontrol Effect of Antagonistic Bacterium B44R-1 against Phytophthora parasitica[J]. Chinese Journal of Biological Control, 2022, 38(6): 1545-1552.

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