Functional Analysis of Temperature Related Pathogenic Gene yqhE in Ralstonia solanacearum

doi:10.16409/j.cnki.2095-039x.2023.03.003

Abstract

Abstract: The protein-protein interoperability (PPI) network analysis of the expression profiles of Ralstonia solanacearum at different temperatures showed that yqhE could be a new temperature related pathogenic gene of Ralstonia solanacearum. the gene yqhE was cloned from Ralstonia solanacearum strain CBM613. The results showed that gene yqhE was 831 bp in length, encoding 276 amino acids. Its function was further confirmed by gene knock-out. The results showed that the pathogenicity of the yqhE^－ mutant cultured at 28 ℃ was decreased and the course of disease was prolonged compared with the wild-type strain. Vitamin C biosynthesis of yqhE^－ mutant cultured at 20 ℃ and 28 ℃ were inhibited. The vitamin C biosynthesis ability of the wild-type strain cultured at 20 ℃ was reduced compared with 28 ℃. Methylglyoxal (MG) and malondialdehyde (MDA) of yqhE^－ mutant significantly accumulated at 20 ℃ and 28 ℃. MG and MDA of the wild-type strain cultured at 20 ℃ accumulated more than at 28 ℃. The inhibition of vitamin C biosynthesis resulted in the weakening of radical scavenging ability and the enhancement of oxidative stress reaction in Ralstonia solanacearum. The accumulation of MG and MDA produced cytotoxicity. The above results could be related to the weakening of pathogenicity in yqhE^－ mutant cultured at 28 ℃ and the loss of pathogenicity in wild-type strain cultured at 20 ℃. In conclusion, yqhE could be a temperature related pathogenic gene in Ralstonia solanacearum.

Key words: Ralstonia solanacearum, temperature related pathogenic gene, yqhE, gene knock-out, functional analysis

CLC Number:

S432.1

ZHOU Daxiang, YANG Junnian, CHEN Hong, YIN Youping, XIONG Shu. Functional Analysis of Temperature Related Pathogenic Gene yqhE in Ralstonia solanacearum[J]. Chinese Journal of Biological Control, 2023, 39(1): 141-148.

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