Screening and Identification of Biocontrol Fungus with High Pathogenicity against Spodoptera litura

doi:10.16409/j.cnki.2095-039x.2023.01.050

Abstract

Abstract: Six strains of entomogenous fungi were isolated and purified from muscardine cadavers of Spodoptera litura larvae,and were identified by morphological and molecular biological methods.The pathogenicity of these strains against S.litura larvae was determined by the dipping method.The six fungal strains were identified as Beauveria bassiana(two strains),Metarhizium rileyi,Fusarium equiseti,Aspergillus nomiae,and Talaromyces trachyspermus,whose LT₅₀ against the pest larvae were 4.737,4.543,1.343,2.086,2.212,and 2.351 days,respectively.All the six strains of entomogenous fungi showed high pathogenicity against the pests at the concentration of 10⁸ conidia/mL,in which M.rileyi established the highest pathogenicity.The present study reports for the first time that S.litura can be infected by F.equiseti and A.nomiae and that T.trachyspermus is able to infect insects.

Key words: Spodoptera litura, entomogenous fungi, pathogenicity

CLC Number:

S476.2

TIAN Yifan, ZHANG Zhengkun, SUI Li, ZHAO Yu, LU Yang, MENG Zhao, SHI Wangpeng, LI Qiyun. Screening and Identification of Biocontrol Fungus with High Pathogenicity against Spodoptera litura[J]. Chinese Journal of Biological Control, 2024, 40(2): 282-290.

References

[1] EFSA Panel on Plant Health (PLH), Bragard C, Dehnen-Schmutz K, et al. Pest categorisation of Spodoptera litura[J]. EFSA Journal, 2019, 17(7):e05765.
[2] 秦厚国,汪笃栋,丁建,等.斜纹夜蛾寄主植物名录[J].江西农业学报, 2006(5):51-58.
[3] 朱俊洪,张方平.香蕉园斜纹夜蛾的发生危害初报[J].中国南方果树, 2005(1):32.
[4] 官宝斌,陈乾锦,陈家骅,等.斜纹夜蛾的生物学和生态学研究[J].华东昆虫学报, 1999(1):57-61.
[5] 杨莹莹,赖晓依,赖多,等. 6种农药对斜纹夜蛾和甜菜夜蛾的杀虫活性测定[J].湖南人文科技学院学报, 2017, 34(6):107-113.
[6] 沈智蓉,殷如龙,浦冠勤.斜纹夜蛾的生物防治研究[J].江苏蚕业, 2007, 115(3):5-8.
[7] 谢建军,胡美英,许再福.斜纹夜蛾的天敌及其生物防治[J].昆虫天敌, 1999(2):82-92.
[8] Inagaki S, Miyasono M, Yamamoto M, et al. Induction of antibacterial activity against Bacillus thuringiensis in the common cutworm, Spodoptera litura(Lepidoptera:Noctuidae)[J]. Applied Entomology and Zoology, 1992, 27(4):565-570.
[9] Inagaki S, Miyasono M, Ishiguro T, et al. Proteolytic processing of δ-endotoxin of Bacillus thuringiensis var. kurstaki HD-1 in insensitive insect, Spodoptera litura:unusual proteolysis in the presence of sodium dodecyl sulfate[J]. Journal of Invertebrate Pathology, 1992, 60(1):64-68.
[10] 符小发,李益,高强,等.球孢白僵菌防治葡萄斜纹夜蛾药效试验[J].农村科技, 2022(5):37-39.
[11] 李世广,林华峰,徐庆丰,等.几种虫生真菌对烟青虫的致病性研究[J].安徽农业大学学报, 2001(4):376-379.
[12] 徐毓笛,魏红爽,石嘉伟,等.三株球孢白僵菌对草地贪夜蛾的毒力比较[J].植物保护学报, 2020, 47(4):867-874.
[13] 李学文,张希鹏,张蕾蕾,等.基于CTAB的莱氏绿僵菌选择性培养基制备及分离效果研究[J].中国植保导刊, 2021, 41(9):22-29, 61.
[14] 裴双康,刘彦妮,弓玉红,等.木贼镰刀菌最适营养条件筛选[J].安徽农学通报, 2020, 26(2):24-26.
[15] 魏景超.真菌鉴定手册[M].上海:上海科学技术出版社, 1979, 650-663.
[16] Kumar S. Mega 3.1:Integrated software for molecular evolutionary genetics analysis and sequence alignment[J]. Briefings in Bioinformatics, 2004, 5(2):150-163.
[17] 庞继鑫,温绍海,杜广祖,等.一株侵染草地贪夜蛾成虫的球孢白僵菌的分离鉴定[J].植物保护, 2022, 48(1):185-190, 203.
[18] 罗成,应盛华,冯明光.球孢白僵菌对斜纹夜蛾高毒菌株筛选与制剂的研发[J].中国生物防治学报, 2011, 27(2):188-196.
[19] 王露露,房耀维,刘姝,等.一株感染家蚕的高致病性白僵菌的筛选及发酵条件优化[J].微生物学通报, 2022, 49(6):2233-2244.
[20] Jafir M, Ahmad J N, Arif M J, et al. Characterization of Ocimum basilicum synthesized silver nanoparticles and its relative toxicity to some insecticides against tobacco cutworm, Spodoptera litura Feb.(Lepidoptera; Noctuidae)[J]. Ecotoxicology and Environmental Safety, 2021, 218:112278.
[21] 张挺峰,王睿,刘长仲.一株侵染豌豆蚜的昆虫病原真菌的分离及鉴定[J].昆虫学报, 2020, 63(1):22-28.
[22] 雷妍圆,章玉苹,薛志洪,等.一株球孢白僵菌的分离鉴定及其对草地贪夜蛾的致病性[J].环境昆虫学报, 2020, 42(3):593-601.
[23] 张胜兰.斜纹夜蛾高毒力棒束孢属菌株的筛选及其致病性研究[D].贵阳:贵州大学, 2020, 21-24.
[24] 杨新军,林华峰,李茂业,等.莱氏野村菌Nr05菌株的培养性状及其对斜纹夜蛾的毒力[J].中国生物防治学报, 2007(1):44-48.
[25] 林华峰.虫生真菌研究进展(综述)[J].安徽农业大学学报, 1998(3):43-46.
[26] Chehri K. Molecular identification of entomopathogenic Fusarium species associated with Tribolium species in stored grains[J]. Journal of Invertebrate Pathology, 2017, 144:1-6.
[27] Ahmad F, Anwar W, Javed M A, et al. Infection mechanism of Aspergillus and Fusarium species against Bemisia tabaci[J]. Mycopath, 2019, 17(2):63-72.
[28] 苑森行,杨玉景.虫生真菌——木贼镰刀菌的初步研究[J].微生物学通报, 1986(1):13-14.
[29] 赵雪怡,柴军发,张暄翊,等.木贼镰刀菌的鉴定及其对桃蚜的致病性测定[J].中国生物防治学报, 2023, 39(1):69-76.
[30] 梁宗琦.昆虫病原真菌的综合开发研究[M]//中国虫生真菌研究与应用(第一卷).北京:学术期刊出版社, 1986, 44-53.
[31] Rivera C S, Blanco D, Oria R, et al. Diversity of culturable microorganisms and occurrence of Listeria monocytogenes and Salmonella spp. in Tuber aestivum and Tuber melanosporum ascocarps[J]. Food Microbiology, 2010, 27(2):286-293.
[32] May T W, Redhead S A, Bensch K, et al. Chapter F of the International Code of Nomenclature for Algae, Fungi, and Plants[M]. Königstein:Koeltz Scientific Books, 2019.
[33] 范瑛阁,龚明福,朱丽霞.黄瓜白粉病菌生防菌筛选及生防机制初步研究[J].北方园艺, 2010(14):150-153.
[34] Naraghi L, Negahban M, Naraghi L. The efficacy of nanoparticles containing Talaromyces Flavus in inhibiting the growth of Verticillium Dahliae, The Causal Agent of Cotton Wilt Disease[J]. International Journal of Agricultural Science and Research, 2018, 8(2):229-240.
[35] Hemmati R, Gholizadeh R. Talaromyces trachyspermus a potential biocontrol agent for branched broom rape (Orobanche ramosa)[J]. Australasian Plant Pathology, 2019, 48(3):1-3.
[36] Farhat H, Urooj F, Irfan M, et al. Biocontrol potential of endophytic fungus Talaromyces trachyspermus against root rot pathogens of sunflower[J]. Research Square, 2021, DOI:https://doi.org/10.21203/rs.3.rs-275521/v1