Silencing of the UDP-glucuronyltransferase Gene (OfUGT) Reverses the Resistance of Ostrinia furnacalis to Cry1Ie

doi:10.16409/j.cnki.2095-039x.2022.11.006

Abstract

Abstract: Bacillus thuringiensis (Bt) genetically modified crops are widely used in pest control, but with long-term planting of Bt genetically modified crops, the target pests will inevitably develop resistance to insecticidal proteins, and how to management the resistance is a difficult problem. Based on the results of previous transcriptome analysis, this study quantitatively analyzed the expression of UDP-glucuronyltransferase gene (OfUGT) in Cry1Ie resistant Asian corn borer strain (ACB-IeR) and susceptible strain (ACB-BtS). The OfUGT gene in ACB-IeR was silenced by RNA interference (RNAi) technology, and the effect of silencing OfUGT gene on ACB-IeR was evaluated by bioassay. The relative expression of the OfUGT gene in ACB-IeR third instar larvae was higher than that in ACB-BtS, and decreased after injection of dsUGT. The lethal concentration of the protein was greater than 1000.00 µg/g in the Cry1Ie-resistant strain. When the protein concentration was 500.00 µg/g, the survival rates of the dsUGT and dsGFP injected larvae did not have significant difference with control group. When the protein concentration was 1000.00 µg/g, the survival rate of the dsGFP injected larvae was lower than control group, and the survival rate of dsUGT injected larvae was significantly lower than the control and dsGFP injected group. The results show that RNAi of the OfUGT gene could reduce the resistance of the resistant line of Asian corn borer to the Cry1Ie protein, but the reversal degree of resistance needs to be further studied.

Key words: Ostrinia furnacalis, UDP-glucuronyltransferase, RNA interference, Cry1Ie, resistance reversal

CLC Number:

S435.132

LIN Yaling, HE Kanglai, WANG Zhenying, SHANG Suqin, ZHANG Tiantao. Silencing of the UDP-glucuronyltransferase Gene (OfUGT) Reverses the Resistance of Ostrinia furnacalis to Cry1Ie[J]. Chinese Journal of Biological Control, 2023, 39(4): 834-841.

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