Effects of Feeding Four Host Plants on Midgut Bacteria of Tuta absoluta

doi:10.16409/j.cnki.2095-039x.2024.02.044

Abstract

Abstract: Tomato leaf miner (Tuta absoluta) is a worldwide pest with a wide range of host species, causing serious harm to its host crops. The mid-gut bacterial diversity and function of its larvae feeding on tomato, potato, eggplant and ginseng, were analyzed by 16S rRNA sequencing. The results showed that 4886, 6016, 2781, and 3471 operational taxonomic units (OTU, respectively) were detected, among which 905 OTUs were common. Alpha and Beta diversity analyses showed mid gut bacterial diversities were the highest in the larvae feeding on potato and eggplant. Species classification analysis showed that, for each host plant, and at each level of classification, mid-gut bacterial species abundance differed. Firmicutes and Proteobacteria were the dominant mid-gut bacterial phyla, Wolbachia was the dominant genus on tomato and potato hosts, and Enterococcus was the dominant genus on ginseng fruit and eggplant hosts. Analyses showed that their metabolic pathways were statistically similar, with the greatest proportion of metabolic pathways for biosynthesis and degradation. In conclusion, though host plant species significantly affect the mid-gut bacterial diversity and community structure of tomato leaf miner larvae, the microbial functions are similar.

Key words: Tuta absoluta, different host plant, midgut bacterium, bacterial diversity

CLC Number:

S476

YANG Yaxian, ZHOU Zhaoxu, QIAN Xiujuan, LIU Yueying, ZHANG Meijiao, JIANG Mingjun. Effects of Feeding Four Host Plants on Midgut Bacteria of Tuta absoluta[J]. Chinese Journal of Biological Control, 2024, 40(4): 776-786.

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