Isolation, Identification and Virulence Analysis of a Nucleopolyhedrovirus Infecting Spodoptera litura

doi:10.16409/j.cnki.2095-039x.2025.09.014

Abstract

Abstract: This study enriched and isolated the pathogens from the dead Spodoptera litura larvae collected from the tobacco fields in Wanqiao Town, Dali Prefecture, Yunnan Province. Through morphological observation, molecular identification, and phylogenetic analysis, the pathogen species was identified. The droplet-feeding method was used to determine the replication characteristics of the pathogen in S. litura and its virulence to the 3^rd instar S. litura larvae. The results showed that the pathogen of the diseased S. litura larvae was S. litura nucleopolyhedrovirus, named SpltMNPV-DL01. This virus strain can replicate rapidly in the larvae of S. litura. When the 3^rd instar S. litura larvae were infected with 1.0×10⁶ PIB/mL virus for 5 days, the genomic copy number of SpltMNPV-DL01 in the body was as many as 8.85× 10⁶ copies/μL. When the 3^rd instar S. litura larvae were inoculated with 1.0× 10⁴, 1.0 ×10⁵, 1.0×10⁶, 1.0×10⁷, and 1.0× 10⁸ PIB/mL nucleopolyhedrovirus, the LT₅₀ values were 7.3 d, 7.0 d, 6.3 d, 5.2 d, and 4.8 d, respectively. The corresponding virulence regression equations were Y＝-3.452+0.474X, Y＝ -3.551+0.508X, Y＝ -3.314+0.524X, Y＝ -3.043+0.560X, and Y＝ -2.722+0.573X, respectively. The LC₅₀ on the 5^th, 6^th, and 7^th days of treatment were 10^7.806, 10^6.285, and 10^4.700 PIB/mL, respectively. On the 8^th day, the cumulative mortality rates of the 3^rd instar S. litura larvae were 72.22%, 75.60%, 84.72%, 91.67%, and 98.61%, respectively. The results provide prospective for the application and development of S. litura nucleopolyhedrovirus.

Key words: Spodoptera litura, nuclear polyhedrosis virus, virulence determination, biological control

CLC Number:

S476.1

YANG Guangyuan, ZHANG Ke, WANG Guang, CAI Haosheng, LI Zhengbin, XIE Yonghui, CHA Chenwen, GU Xiaofei, CHEN Bin. Isolation, Identification and Virulence Analysis of a Nucleopolyhedrovirus Infecting Spodoptera litura[J]. Chinese Journal of Biological Control, 2026, 42(1): 208-217.

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