Investigation of Endophytic Fungal Strains 38L1 and G3-23 as Seed Priming Bioagents for Biocontrol and Growth Enhancement in Wheat

doi:10.16409/j.cnki.2095-039x.2026.02.018

Abstract

Abstract: Seed bio-priming is a simple and cost-effective technique that enhances crop performance by improving seed germination and seedling establishment. In this study, we investigated the potential of seed bio-priming with endophytic fungi Epicoccum nigrum 38L1 and Fusarium acuminatum G3-23 to promote wheat growth and suppress Fusarium head blight(FHB) and crown rot(FCR), with a particular focus on the underlying defense mechanisms. The results showed that priming with both strains significantly enhanced agronomic traits such as plant height, effective tiller number, kernel number per spike, root length and root weight. Moreover, primed plants displayed strong disease resistance, achieving maximum control efficacies of 45.45% against FHB and 50.84% against FCR. Measurement of defense-related enzyme activities in wheat leaves revealed that both treatments significantly induced the activities of PAL, POD, and SOD, suggesting an enhanced defensive response and improved disease resistance in plants. By concurrently promoting growth and activating defense mechanisms, seed bio-priming with strains 38L1 and G3-23 emerges as an effective strategy for the integrated management of FHB and FCR in wheat. This dual-action approach provides a sustainable solution for disease control and underscores the potential of these endophytic fungi as promising biocontrol agents for green agriculture.

Key words: seed priming, endophytic fungi, wheat Fusarium head blight, wheat crown rot, growth promotion

CLC Number:

S476

YUAN Chengke, Clement Nzabanita, AN Qi, WANG Yanfei, WANG Shuangchao, GUO Lihua, FANG Shouguo. Investigation of Endophytic Fungal Strains 38L1 and G3-23 as Seed Priming Bioagents for Biocontrol and Growth Enhancement in Wheat[J]. Chinese Journal of Biological Control, 2026, 42(3): 591-599.

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