Influences of Bacillus amyloliquefaciens TR2 on Soil Enzyme Activities and Its Effects on Disease Control and Growth Promotion in Strawberry

doi:10.16409/j.cnki.2095-039x.2022.02.014

Abstract

Abstract: In order to explore the soil microecological mechanisms of biocontrol bacteria in disease prevention and growth promotion, the strawberry plants were inoculated with Bacillus amyloliquefaciens TR2 through root-drenching method in this study. The soil enzyme activities in different periods were analyzed by use of colorimetry and titration, and the strawberry growth and the incidence of strawberry root rot were investigated. The results showed that the activities of urease, sucrase, catalase and phosphatase in the rhizosphere soil of the strawberry plants were significantly higher than those in the control group after inoculating with B. amyloliquefaciens TR2, and the promotion lasted for a long time until the 60^th day. The urease and sucrase activities were increased in the strawberry rhizosphere soil treated with B. amyloliquefaciens TR2, and the significant promoting effect were detected from the 7^th and 15^th day respectively, and reached the maximum on the 30^th day, as that the activity of urease and sucrase was 215% and 124% higher than that of the control, respectively. The phosphatase and catalase activities were also dramatically enhanced by inoculation of B. amyloliquefaciens TR2 from the 15^th day and 30^th day, respectively. The increase of the catalase activity reached a peak on the 60^th day, which was 43% higher than that of the control. While, enhancement of the phosphatase activity was the highest on the 15^th day as that the activity of acid phosphatase and alkaline phosphatase increased by 69% and 45%, respectively, compared with that in the control group. Growth of the strawberry plants were improved by treating with B. amyloliquefaciens TR2, as that root length, root weight, leaf area and maximum of single fruit weight were better than those in the control group. B. amyloliquefaciens TR2 increased the root length by about 20% and root weight by 92% compared with that in the control group on the 60^th day and the average maximum leaf area was 10 cm² bigger than that in the control group on the 15^th day. Besides, each plant produced 1.2 more leaves than that of the control on the 30^th day. The incidence of root rot in the experimental group treated with B. amyloliquefaciens TR2 was reduced by about 25.0%.

Key words: strawberry, Bacillus amyloliquefaciens, soil enzyme activity, growth promotion, disease prevention

CLC Number:

S476

ZHAO Yumeng, MIAO Peipei, WANG Xudong, GAO Tantan, SHI Xiaoyi, JIA Shaolei, REN Zhengguang, ZHAO Xiaoyan. Influences of Bacillus amyloliquefaciens TR2 on Soil Enzyme Activities and Its Effects on Disease Control and Growth Promotion in Strawberry[J]. Chinese Journal of Biological Control, 2022, 38(2): 495-501.

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