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Influence of Temperature, Moisture, and Soil Type on Conidium Germination of Metarhizium pingshaense in Soil

WU Xiaoshuang, ZHANG Yabo, WU Panpan, YE Bihuan, WANG Haojie, SHU Jinping   

  1. Institute of Subtropical Forestry, Chinese Academy of Forestry Siences, Fuyang 311400, China
  • Received:2014-02-21 Revised:1900-01-01 Online:2014-12-08 Published:2014-12-08

Abstract: The conidia germination of Metarhizium pingshaense is an important factor to control soil pests. The effects of soil temperature, moisture and type on conidia germination and the life cycle of M. pingshaense in soil were investigated in this research. The results showed the main factors of conidia germination were soil temperature and moisture, followed by soil type. Under the most optimum temperature and humidity condition, the conidia germination ratios were more than 80.0% in bamboo forest soil, farm soil and riverside soil. The optimum temperature and moisture for conidia germination were different in different soil types. In bamboo forest soil, the conidia germination rate increased with soil humidity and reached 80.8% with moisture of 24% at the most optimum temperature of 25 ℃. In farm soil, the conidia germination rate was negative correlation with the moisture and reached 86.5% with moisture of 6% at the most optimum temperature of 25 ℃. The most optimum temperature for conidia germination was 30 ℃ in riverside soil. The conidia germination rate was negative correlation with the moisture and reached 80.0% when the moisture was 6%. The life cycle of M. pingshaense in soil was observed at 25 ℃ and with moisture of 12%. The results showed that germ tubes was produced at 12 h, 24—96 h for the vegetative growth stage, 120—144 h for reproductive growth stage and then initiation of sporulation, catenulate conidia observed at 168 h.

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