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journal1 ›› 2016, Vol. 32 ›› Issue (1): 33-39.DOI: 10.16409/j.cnki.2095-039x.2016.01.005

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Construction and Analysis of Suppression Subtractive Hybridization cDNA Library of the Diapause Coccinella septempunctata L.

JIANG Sha1,2, Huang Fengxia2, Qi Xiaoyang1, Ren Xiaoyun2, CHEN Hongyin2, HUANG Jian1, ZHANG Lisheng2   

  1. 1. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture/Institute of Plant Protection, Chinese Academy of Agricultural Sciences/Sino-American Biological Control Laboratory, Beijing 100193, China
  • Received:2015-03-18 Online:2016-02-08 Published:2016-02-22

Abstract: The ladybird Coccinella septempunctata L., an excellent insect natural enemy, enter diapause to avoid unfavorable environmental conditions, which offers a mechanism for a survival. In this study, suppression subtractive hybridization (SSH) library and reverse Northern hybridization blot were employed to screen differentially expressed genes in the diapause insects and 231 differentially dots were obtained. Using an approach of blastx/n alignment, 64 and 54 differentially expressed genes were identified in forward and reverse library, respectively. These genes were mainly involved in signal transduction, biosynthesis, primary metabolism and secondary metabolism. Multiple genes found in the library provide references for further screening, cloning, and analysis of diapause-associated genes. The biological functions of 28 kD desiccation stress protein associated with cold hardiness, Cullin 3 related to the development of ovary and isocitrate dehydrogenase involved in energy metabolism were discussed in particular and their relatedness with diapause in C. septempunctata was reviewed.

Key words: Coccinella septempunctata, suppression subtractive hybridization, differentially expressed genes, reverse Northern dot-blotting, diapause

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