Welcome to Chinese Journal of Biological Control,Today is

Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (5): 811-820.DOI: 10.16409/j.cnki.2095-039x.2020.05.023

• RESEARCH REPORTS • Previous Articles     Next Articles

Screening and Identification of Endophytic Bacteria Isolated from Root Nodules for Control of Meloidogyne incognita

ZHAO Jinjie1, WANG Shuai1, FAN Haiyan1, ZHAO Di2, LIU Xiaoyu3, ZHU Xiaofeng1, WANG Yuanyuan4, DUAN Yuxi1, CHEN Lijie1   

  1. 1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China;
    2. Analytical and Testing Center, Shenyang Agricultural University, Shenyang 110866, China;
    3. College of Science, Shenyang Agricultural University, Shenyang 110866, China;
    4. College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2020-01-14 Online:2020-10-08 Published:2020-11-20

Abstract: Root-knot nematode causes dramatic yield losses in many crops. Biocontrol agents, which are eco-friendly and generally non-toxic, provide an effective strategy for root-knot nematode disease control. In this study, three bacteria strains Sneb1994, Sneb1995, and Sneb2000 showing biocontrol potential against Meloidogyne incognita were screened from more than 400 endophytic bacterial isolated from root nodules. The strain fermentation filtrates of Sneb1994, Sneb1995, and Sneb2000 showed high nematocidal activity against M. incognita second-stage juveniles (J2), with corrected mortalities of more than 85% at 24 hours post treatment. Furthermore, the strains had inhibitory effect on nematode egg hatching and promotional effect on the growth of tomato seeds. The results of pot experiments indicated that root treatment with fermentation filtrates of the three strains significantly reduced root galls and egg masses, with the relative biocontrol efficacies of 37.95%-44.51%, and promoted the growth of tomato plants. Strains Sneb1994, Sneb1995, and Sneb2000 were identified as Microbacterium oxydans, Pseudomonas brassicacearum, and Bacillus cereus, respectively, based upon morphological, physiological and biochemical characteristics, and 16S rDNA sequence analysis. This is the first report of M. oxydans as a biocontrol agent against M. incognita. These endophytic bacteria isolated from root nodules provide new microbial resource for the biological control of plant-parasitic nematode disease.

Key words: Meloidogyne incognita, root nodule endophytic bacteria, identification, control effect, tomato

CLC Number: