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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (1): 242-249.DOI: 10.16409/j.cnki.2095-039x.2021.08.006

• RESEARCH REPORTS • Previous Articles     Next Articles

Biocontrol Effect of Strain CSR-2 on Maize Stalk Rot and Complete Genome Sequence Analysis

ZHAO Meixi, BAI Zhongyi, ZHANG Xianghui, LIU Jinliang, PAN Hongyu, ZHANG Yanhua   

  1. College of Plant Science, Jilin University, Changchun 130062, China
  • Received:2021-08-30 Online:2022-02-08 Published:2022-03-01

Abstract: A potential biocontrol strain, CRS-2, was isolated from maize rhizosphere soil. The strain showed antagonism against Fusarium graminearum, Magnaporthe oryzae, Botrytis cinerea, Sclerotinia sclerotiorum, Cercospora zeae, Helminthosporium maydis, and Rhizoctonia solani. The strain CRS-2 could inhibit the mycelial growth, mycelial morphology and spore germination of F. graminearum. The experiment of combined CRS-2 and water and fertilizer under film drip irrigation showed that the field control efficacy of CRS-2 against maize stalk rot was 52.3%. The complete genome sequence of CRS-2 were obtained by the third-generation PacBio sequencing technology. The genome length was 3202366 bp, encoding 3007 genes with average gene length gene was 943.76 bp. A total number of 198692 high-quality long reading fragments were obtained, the circle genome map was constructed, and the annotated coding genes showed that the strain was Asaia bogorensis (GenBank accession number:CP081941). This research provides a theoretical basis for further discovering microbial resources and their metabolic gene clusters for agricultural biological control.

Key words: biocontrol strain, maize stalk rot, Fusarium graminearum, genome sequencing

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