Screening and Identification of Antagonistic Bacteria of Maize Ear Rot and Its Degradation Effect on DON

doi:10.16409/j.cnki.2095-039x.2021.06.005

Abstract

Abstract: Ear rot is an important disease in maize agricultural production. While causing yield loss, the toxins produced by the pathogens bring serious harms to human and animal health. In order to obtain the biocontrol strain against maize ear rot with the function of degrading toxins, DON was used as the target, and a bacterial strain TP capable of degrading this toxin was isolated and screened from maize diseased ears in field. Based on colony morphology and 16S rDNA and gyrB gene sequence analysis, the strain TP was identified as Bacillus velezensis. Confrontation and cocultivation tests showed that the inhibition rates of TP against Fusarium graminearum F18 reached 70.63% and 99.5%, respectively. After the strain TP was cultured in the inorganic salts solution containing DON for 10 days, the degradation rate of DON was 98.97%. The above results showed that the strain TP could inhibit the growth of F. graminearum F18 and degrade DON efficiently. It has a promising application prospects in controlling maize ear rot and reducing the derived toxin hazard caused by F. graminearum.

Key words: DON, degradation, Bacillus velezensis, Fusarium graminearum

CLC Number:

S476

ZHOU Hongzi, ZHOU Fangyuan, DUAN Chengding, WU Xiaoqing, ZHAO Xiaoyan, XIE Xueying, ZHANG Guangzhi, FAN Susu, ZHANG Xinjian. Screening and Identification of Antagonistic Bacteria of Maize Ear Rot and Its Degradation Effect on DON[J]. Chinese Journal of Biological Control, 2021, 37(5): 1016-1023.

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