Isolation, Identification of Antagonistic Bacteria AX-3 against Fusarium graminearum and Identification of Its Antagonistic Substance

doi:10.16409/j.cnki.2095-039x.2020.01.008

Abstract

Abstract: Fusarium graminearum can cause Fusarium Head Blight (FHB) which seriously threatens the quality and safety of wheat in China. In this study, from the perspective of biological control, we want to isolate antagonistic bacteria, study the antagonistic characteristics and use it in the biological control of FHB. Antagonistic AX-3 against Fusarium graminearum was isolated from wheat samples by serial dilution and confrontation culture. According to phylogenetic tree constructed by 16S rDNA gene and gyrB gene sequences and physiological and biochemical characteristics, strain AX-3 was identified as Bacillus amyloliquefaciens. The antagonistic spectrum of AX-3 was studied by confrontation culture, and the results showed that strain AX-3 had strong antagonistic activities against many species of Fusarium pathogens, and its bacteriostatic circle radius is 150-280 mm, which indicating that AX-3 had broad-spectrum antagonistic properties. The antagonists in the strain AX-3 were separated and purified by column chromatography and preparative liquid chromatography, and then their structures were identified by high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. The results showed that one of the antagonistic substances produced by Bacillus amyloliquefaciens AX-3 was macrolactin A.

Key words: Fusarium graminearum, Bacillus amyloliquefaciens, antagonistic substance

CLC Number:

S476

XU Limei, HE Dan, WANG Gang, XU Shengjia, SHI Jianrong, XU Jianhong. Isolation, Identification of Antagonistic Bacteria AX-3 against Fusarium graminearum and Identification of Its Antagonistic Substance[J]. Chinese Journal Of Biological Control, 2020, 36(1): 125-134.

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