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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (1): 125-134.DOI: 10.16409/j.cnki.2095-039x.2020.01.008

• RESEARCH REPORTS • Previous Articles     Next Articles

Isolation, Identification of Antagonistic Bacteria AX-3 against Fusarium graminearum and Identification of Its Antagonistic Substance

XU Limei1,2, HE Dan2, WANG Gang2, XU Shengjia1,2, SHI Jianrong2, XU Jianhong1,2   

  1. 1. Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture/College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;
    2. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation(Nanjing), Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2019-04-16 Online:2020-02-08 Published:2020-02-26

Abstract: Fusarium graminearum can cause Fusarium Head Blight (FHB) which seriously threatens the quality and safety of wheat in China. In this study, from the perspective of biological control, we want to isolate antagonistic bacteria, study the antagonistic characteristics and use it in the biological control of FHB. Antagonistic AX-3 against Fusarium graminearum was isolated from wheat samples by serial dilution and confrontation culture. According to phylogenetic tree constructed by 16S rDNA gene and gyrB gene sequences and physiological and biochemical characteristics, strain AX-3 was identified as Bacillus amyloliquefaciens. The antagonistic spectrum of AX-3 was studied by confrontation culture, and the results showed that strain AX-3 had strong antagonistic activities against many species of Fusarium pathogens, and its bacteriostatic circle radius is 150-280 mm, which indicating that AX-3 had broad-spectrum antagonistic properties. The antagonists in the strain AX-3 were separated and purified by column chromatography and preparative liquid chromatography, and then their structures were identified by high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. The results showed that one of the antagonistic substances produced by Bacillus amyloliquefaciens AX-3 was macrolactin A.

Key words: Fusarium graminearum, Bacillus amyloliquefaciens, antagonistic substance

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