Comparative Transcriptomic Profiles Revealed JA- and SA- Regulated Soft Rot Disease Resistance by Bacillus velezensis BCP6 in Amorphophallus konjac

doi:10.16409/j.cnki.2095-039x.2024.02.060

Abstract

Abstract: The application of Bacillus velezensis has been proven to significantly enhance the resistance to soft rot in Amorphophallus konjac, yet its precise biological control mechanisms remain unclear. This study explored the effects of B. velezensis BCP6 on the defense pathways of A. konjac in response to P. aroidearum MY11 infection through comparative transcriptome analysis. Differential expression analysis, gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) revealed that BCP6 application upregulated pathways related to jasmonic acid (JA), reactive oxygen species, and cell wall synthesis, thereby activating the basic defense system of A. konjac. This immune activation facilitated the rapid initiation of defense responses when subjected to subsequent P. aroidearum infection by enhancing the JA-mediated induced systemic resistance (ISR) and SA-dependent systemic acquired resistance (SAR) signaling pathways. Simultaneously, a significant increase in the expression of genes involved in cell wall formation and pathogenesis-related proteins was observed, effectively enhancing resistance to soft rot. Meanwhile, exogenous JA treatment was found to directly stimulate the expression of cell wall-related genes, namely CESA2 and CSI1, while SA significantly induced the expression of SA-dependent defense genes TLP and E13C. The results provided a theoretical basis for biological control and disease-resistant breeding of konjac.

Key words: Bacillus velezensis, soft rot resistance, RNA-seq, inducted systemic resistance ISR, systemic acquired resistance SAR

CLC Number:

S476

ZHANG Meng, CHEN Sen, TANG Dengguo, HUANG Yan, LONG Zhijian, WANG Boya, HU Shanglian, CAO Ying. Comparative Transcriptomic Profiles Revealed JA- and SA- Regulated Soft Rot Disease Resistance by Bacillus velezensis BCP6 in Amorphophallus konjac[J]. Chinese Journal of Biological Control, 2024, 40(6): 1375-1385.

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