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Chinese Journal of Biological Control ›› 2023, Vol. 39 ›› Issue (6): 1434-1445.DOI: 10.16409/j.cnki.2095-039x.2023.02.068

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Inhibition Effect and Transcriptome Analysis of Isooctyl alcohol on Botrytis cinerea

DUAN Tiankun1, WANG Yan1, YUAN Jiaqi1, SU Yaxin1, SHI Luxin1, WANG Lin2, SU Jian2, WANG Meiqin1, WANG Chunwei1   

  1. 1. College of Agronomy, Shanxi Agricultural University, Taigu 030801, China;
    2. Taiyuan Customs District, Taiyuan, Shanxi 030021, China
  • Received:2023-07-11 Online:2023-12-08 Published:2023-12-20

Abstract: To reveal the inhibitory mechanism of isooctyl alcohol to Botrytis cinerea, the inhibition effect of isooctyl alcohol on B. cinerea was determined using two-sealed-base-plate method. Mycelial morphology treated with isooctyl alcohol was observed by scanning electron microscopy. The influence of isooctyl alcohol on the extracellular conductivity and the ergosterol synthesis were measured. Response of B. cinerea to isooctyl alcohol was analyzed by RNA sequencing (RNA-Seq). The results showed that the inhibition rate of isooctyl alcohol to mycelia was 67.92%. After treatment with isooctyl alcohol, lots of mycelia were deformed and distorted with the increasing of membrane permeability. At 4, 5 and 6 days after isooctyl alcohol treatment, the ergosterol contents in cell membrane decreased to 44.35%, 38.82% and 42.43%, and the protein concentration decreased to 65.70%, 77.93% and 58.57%, respectively. A total of 577 differentially expressed genes (DEGs), including 312 up-regulated genes and 265 down-regulated genes, were obtained by RNA-Seq. Based on functional analysis of clusters of orthologous groups (COG), gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG), DEGs involved in membrane integral components, ergosterol biosynthesis pathway, amino acid metabolism and ribosome biogenesis pathway were significantly down-regulated, indicating that isooctyl alcohol might affect ergosterol content, energy production and ribosome structure, and further play inhibit role in the growth of B. cinerea.

Key words: isooctyl alcohol, Botrytis cinerea, inhibition effect, RNA-Seq

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