Identification of the Biocontrol Bacterium YB and Biocontrol Effect and Action Mechanism on Pumpkin Fusarium disease

doi:10.16409/j.cnki.2095-039x.2026.02.038

Abstract

Abstract: To obtain effective biocontrol strains against Fusarium oxysporum caused pumpkin Fusarium wilt, the antifungal activity and control efficacy of a biocontrol strain YB, isolated from the soil in the previous experiment, was conducted and evaluated in this study. The taxonomic status of the YB strain was clarified, and its biocontrol mechanism was preliminarily explored. Based on the analysis of morphological characteristics, physiological and biochemical properties, as well as phylogenetic analysis of 16S rRNA and gyrB sequences, the strain YB was identified as Bacillus velezensis. Strain YB exhibited potent inhibitory activity against eight pathogenic fungi in plate confrontation assays, achieving the highest inhibition rate(83.63%) against Fusarium oxysporum. The stock solution of the fermentation filtrate of strain YB had the strongest inhibitory effect on the germination of conidia of Fusarium oxysporum, with an inhibitory rate of 90.49%. In the pot experiment, the control efficacy of inoculation with the fermentation broth of YB strain for 24 hours followed by inoculation with F. oxysporum(protective effect) was 83.45%, while the control efficacy of inoculation with the fermentation broth of strain YB for 24 hours followed by inoculation with F. oxysporum(therapeutic effect) was 62.94%. These results indicate that B. velezensis YB strain has obvious control effects on pumpkin Fusarium wilt, and the control effect of the protective effect is better than that of the therapeutic effect. In the therapeutic pumpkin plants, the activities of SOD, PAL, CAT and PPO reached the maximum at 48 h, which were 1.6, 2.74, 3.25 and 4.65 times those of the sterile water treatment, respectively. In pumpkin plants with the protective effect, the activities of the four enzymes peaked at 72 h, reaching 1.7, 2.55, 5.28, and 4.36 times those of the sterile water treatment, respectively. In conclusion, B. velezensis YB effectively controls pumpkin Fusarium wilt and provides the excellent microbial resource for biological control of Fusarium wilt.

Key words: pumpkin Fusarium wilt, Bacillus velezensis, biological control, pot experiment

CLC Number:

S476

LIU Siyu, HEI Yaya, XUE Yingyu. Identification of the Biocontrol Bacterium YB and Biocontrol Effect and Action Mechanism on Pumpkin Fusarium disease[J]. Chinese Journal of Biological Control, 2026, 42(3): 695-708.

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