Isolation and Identification of Anthracnose Pathogens in Devil Pepper and Screening of Biocontrol Bacillus Strains

doi:10.16409/j.cnki.2095-039x.2026.02.016

Abstract

Abstract: The devil pepper fruits with typical anthracnose symptoms were collected from Dehua County, Quanzhou City, Fujian Province. The pathogens were isolated by using the tissue isolation method and identified by using a multi-locus phylogenetic tree of ITS-CHS-GAPDH, combining with morphological characteristics and Koch's rule. Meanwhile, the antagonistic Bacillus strains against the anthracnose pathogens were screened via the antimicrobial zone method. The results showed that a total of 26 pathogen isolates were obtained, 19 and 7 of them were identified as Colletotrichum truncatum and Colletotrichum scovillei, with isolation frequencies of 73.08% and 26.92%, respectively. Moreover, compound infections by C. truncatum and C. scovillei were found in some pepper fruits. Subsequently, 14 and 15 Bacillus strains with antagonistic effects against C. truncatum and C. scovillei were obtained by inhibition zone tests, respectively. The results of the pot experiments showed that the control efficacies of the above-mentioned antagonistic Bacillus strains against the pepper anthracnose caused by C. truncatum and C. scovillei were 7%-73.68% and 10.78%-99.02%, respectively. Moreover, 5 antagonistic Bacillus strains had more than 70% of control efficacies against the pepper anthracnose caused by C. scovillei, and that of the strain B. subtilis BS-11709 achieved up to 99.02%. Additionally, only the strain B. subtilis BS-10566 exhibited good control efficacies against the pepper anthracnose caused by both C. truncatum and C. scovillei, being 73.68% and 82.35%, respectively. This study provided new Bacillus strain resources for biocontrol of the pepper anthracnose.

Key words: pepper, anthracnose, Colletotrichum truncatum, Colletotrichum scovillei, biocontrol bacterium, Bacillus

CLC Number:

S476

YANG Chen, CHEN Xing, ZHANG Ya, CHEN Yanping, LIU Shuangqing, WANG Jieping. Isolation and Identification of Anthracnose Pathogens in Devil Pepper and Screening of Biocontrol Bacillus Strains[J]. Chinese Journal of Biological Control, 2026, 42(3): 685-694.

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