Molecular Mechanisms of Induced Resistance to Tomato Pathogens by Endophytic Bacterium TF28

doi:10.16409/j.cnki.2095-039x.2015.06.014

Abstract

Abstract: The objective of this study is to elucidate molecular mechanisms of induced resistance to tomato pathogens by endophytic bacterium TF28. In this study, relatively quantitative RT-PCR method was used to analyze the impact of endophytic bacterium TF28 on the expression of MAPK and WRKY gene. Proteomics technology, including 2-D electrophoresis, mass spectrometry and bioinformation analysis, were used to analyze different proteins in tomato leaves between control and TF28 treatment. Results indicated that the expression of MAPK and WRKY gene in tomato leaves were up-regulated after inoculation with TF28. Expression of WRKY gene was higher than that of MAPK. Both two genes reached the maximum expression in leaves at 48 h after inoculation. A total of 373 proteins were obtained using 2-D electrophoresis, of which 18 showed up-regulated, 53 down-regulated and 23 specific expression in tomato leaves after inoculation with strain TF28 two days later, but 280 showed identical to the control. Mass spectrometry analysis and functional annotations were performed for 14 specific expression proteins and the results revealed that 9 was related to disease defense, 3 correlated with quality and 2 reffered to growth regulation.

Key words: endophytic bacterium, tomato, defense signal, MAPK and WRKY gene, expression, different proteins

CLC Number:

S476

ZHANG Shumei, LI Jing, JIANG Wei, MENG Liqiang, CHEN Jingyu, HU Jihua, CAO Xu, YIN Deliang. Molecular Mechanisms of Induced Resistance to Tomato Pathogens by Endophytic Bacterium TF28[J]. journal1, 2015, 31(6): 913-920.

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