Evaluation of Transgenic cry1Ab+cry1C Rice Lines for Its Resistance to Chilo suppressalis

doi:10.16409/j.cnki.2095-039x.2018.01.008

Abstract

Abstract: Control efficacies of four transgenic rice lines, cry1Ab, cry1C, cry1Ab+cry1C and cry1C+cry1Ab, against the main target pest, Chilo suppressalis, were evaluated through detached rice tissues bioassay, and Cry1Ab and Cry1C protein contents in these transgenic lines were measured by enzyme-linked immunosorbent assays. The results showed cumulative feeding areas of C. suppressalis feeding on transgenic rice lines for 48 h and 96 h were significantly lower than those on non-transgenic rice line, MH63. Four insect-resistant transgenic rice lines presented very high resistant level against C. suppressalis at early developmental stage (seedling, tillering and elongation stages), while control efficacy of transgenic lines obviously decreased at later growth stages (booting and maturing stages). Double insect-resistant genes pyramided rice lines exhibited the highest resistant level among the four transgenic lines, second followed by cry1Ab and cry1C lines. Both Cry1Ab and Cry1C protein levels showed significantly different in respective transgenic rice lines at five different developmental stages. Additively, Cry1Ab protein expressing levels in transgenic lines were significantly higher than Cry1C protein. Cry1Ab levels were shown firstly increased and then decreased with the increase of growth stages in single-gene line, however, they presented the gradually decreasing trend with the increasing developmental stages both in leaf and stem tissues in double-gene lines. Cry1C levels showed the gradually rising trend with the increasing developmental stages in leaf tissues both in single-and double-gene lines. Moreover, Cry1Ab or Cry1C levels did not exhibit the significant decrease in double-gene lines, when compared with that in single-gene lines. Therefore, two-gene pyramided plants have robust prospects for commercial use, because they not only provide the efficient control against the pests, but also delay the insect resistance evolution. These results will provide the scientific data and theoretical basis for environmental safety assessment of transgenic Bt crops.

Key words: Chilo suppressalis, insect-resistant transgenic rice, control efficacy, insecticidal protein expression

CLC Number:

S435.112

CHEN Geng, HE Shan, HAN Lanzhi, PENG Yufa. Evaluation of Transgenic cry1Ab+cry1C Rice Lines for Its Resistance to Chilo suppressalis[J]. journal1, 2018, 34(1): 71-78.

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