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Screening of Higher Virulent Fungal Strain against Delia antigua

YUAN Lei, SHEN Ling, WANG Zhongkang, YIN Youping   

  1. Chongqing Engineering Research Center for Fungal Insecticides/College of Life Science, Chongqing University, Chongqing 400030, China
  • Received:2015-08-03 Online:2016-04-08 Published:2016-04-08

Abstract: Six strains of entomopathogenic Beauveria bassiana were screened depending on virulence against Delia antigua. The virulence of these strains against D. antigua were further evaluated in laboratory. Bioassay results showed that the strain CQBb119 had the highest virulence. The accumulative mortality reached 77.78% and the cadaver rate reached 58.52%. The LT50 value was 6.10 d when D. antigua maggot were treated with 1×107 spores/mL spore suspension for 14 d. Inoculated on maggot, pupa, and onion fly of the D. antigua, the test results indicated that the mortality of D. antigua increased with the concentration of the spores suspension while the median lethal time fell. When treated with 1×108 and 1×109 spores/mL spores suspension of CQBb119, LT50 values of D. antigua maggot were 5.48 d and 4.94 d. The LC50 value at 14th day was 1.35×106 spores/mL. B. bassiana CQBb119 had a certain virulence against the onion fly and pupa but the mortality was lower than 50%. The results indicated that the screened entomopathogenic B. bassiana CQBb119 had effective insecticidal activity, and could be used as a potential biological pesticide for controlling of D. antigua.

Key words: Beauveria bassiana, Delia antigua, virulence, biocontrol

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