Physiological Effects of Metarhizium flavoviride on Brown Planthopper (Nilaparvata lugens Stål)

doi:10.16409/j.cnki.2095-039x.2018.05.008

Abstract

Abstract: In the present study, the effects of infection by Metarhizium flavoviride on contents of carboxylesterase (CarE), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), peroxidase (PO), uric acid and 6 essential amino acids in the brown planthopper Nilaparvata lugens Stål were investigated. Three concentrations (1.0×10⁷, 1.0×10⁸ and 1.0×10⁹ spores/L) of M. flavoviride solution were treated by spraying to the planthopper. The results showed that the contents of CarE and GST in the M. flavoviride-treated N. lugnes were significantly lower than those in the untreated N. lugens at 72 h after spraying. CAT and SOD contents in N. lugens increased over time after M. flavoviride spraying. The contents of SOD in the M. flavoviride-treated N. lugnes were 430.87 U/mg, 377.64 U/mg and 376.16 U/mg for the three spraying concentrations, respectively, at 72 h after treatment, which were significantly higher than those in the untreated N. lugens. The contents of PO were not significantly different among treatments at 24 h and 48 h after treatment. However, at 72 h after spraying with 1.0×10⁹ spores/L M. flavoviride, the contents of PO in the M. flavoviride-treated N. lugnes increased to 316.43 U/mg, significantly higher than those in the control N. lugnes. The results also indicated spraying with 1.0×10⁷ spores/L M. flavoviride significantly increased the accumulation of uric acid. The amino acids showed no significant decrease in the M. flavoviride-treated N. lugens. But the levels of lysine (Lys) and threonine (Thr) were as high as 204.68 μg/g and 112.38 μg/g, respectively, at 72 h after spraying with 1.0×10⁷ spores/L M. flavoviride, significantly higher than those in the control N. lugens. In summary, the infection of M. flavoviride affects the detoxification enzymes, protective enzymes and the content of amino acids in N. lugens.

Key words: Metarhizium flavoviride, Nilaparvata lugens, detoxification enzyme, protective enzyme, phenol oxidase, uric acid, amino acid

CLC Number:

S476.1

ZHANG Juefeng, CHEN Jianming, SHU Jingping, LI Fang, ZHONG Haiying, WU Hong. Physiological Effects of Metarhizium flavoviride on Brown Planthopper (Nilaparvata lugens Stål)[J]. journal1, 2018, 34(5): 701-707.

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