Welcome to Chinese Journal of Biological Control,Today is

journal1 ›› 2016, Vol. 32 ›› Issue (6): 756-761.DOI: 10.16409/j.cnki.2095-039x.2016.06.012

• RESEARCH REPORTS • Previous Articles     Next Articles

Effects of Different Inhibitors on Digestive Enzyme and Detoxification Enzyme in Midgut of Locusta migratoria manilensis during Metarhizium anisopliae Infection

WANG Zhenghao1,2,3, JIA Miao2,3, ZHANG Yanfeng2,3, DU Guilin4, CAO Guangchun2,3, NONG Xiangqun2,3, LIU Changzhong1, WANG Guangjun2,3, ZHANG Zehua2,3   

  1. 1. College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China;
    2. State Key Laboratory of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    3. Scientific Observation and Experimental Station of Pests in Xilingol Rangeland, Ministry of Agriculture, Xilinhot 026000, China;
    4. National Animal Husbandry Service, Beijing 100125, China
  • Received:2016-04-10 Online:2016-12-08 Published:2016-12-07

Abstract: To investigate the infection mechanism of Metarhizium anisopliae in midgut of Locusta migratoria manilensis (Meyen), effects of M. anisopliae on the activities of main digestive enzymes and detoxification enzymes in locust midgut were measured. The activities of total protease, trypsin like and chymotrypsin like enzymes, acetylcholinesterase (AChE) and glutathione-S-transferases (GSTs) were assayed to L. migratoria manilensis fed with M. anisopliae, trypsin like enzyme inhibitor (APMSF) and chymotrypsin like enzyme inhibitor (TPCK). In the M. anisopliae treated group, the total activity of protease experienced increase soon after treatment, decrease and increase again in comparison with the control within 10 days. Activities of trypsin like enzymes decreased sharply followed by a great increase first and activities of chymotrypsin like enzymes increased gradually during the whole period. The two inhibitors, especially TPCK, reduced the activities of total protease and two detoxification enzymes on the whole and suppressed the infection of M. anisopliae in the midgut of L. migratoria manilensis. These results reveal the interaction relation between M. anisopliae and hosts and the immune mechanism of hosts against pathogens.

Key words: Metarhizium anisopliae, Locusta migratoria manilensis, inhibitor, digestive enzymes, detoxification enzymes

CLC Number: