Effects of Different Inhibitors on Digestive Enzyme and Detoxification Enzyme in Midgut of Locusta migratoria manilensis during Metarhizium anisopliae Infection

doi:10.16409/j.cnki.2095-039x.2016.06.012

Abstract

Abstract: To investigate the infection mechanism of Metarhizium anisopliae in midgut of Locusta migratoria manilensis (Meyen), effects of M. anisopliae on the activities of main digestive enzymes and detoxification enzymes in locust midgut were measured. The activities of total protease, trypsin like and chymotrypsin like enzymes, acetylcholinesterase (AChE) and glutathione-S-transferases (GSTs) were assayed to L. migratoria manilensis fed with M. anisopliae, trypsin like enzyme inhibitor (APMSF) and chymotrypsin like enzyme inhibitor (TPCK). In the M. anisopliae treated group, the total activity of protease experienced increase soon after treatment, decrease and increase again in comparison with the control within 10 days. Activities of trypsin like enzymes decreased sharply followed by a great increase first and activities of chymotrypsin like enzymes increased gradually during the whole period. The two inhibitors, especially TPCK, reduced the activities of total protease and two detoxification enzymes on the whole and suppressed the infection of M. anisopliae in the midgut of L. migratoria manilensis. These results reveal the interaction relation between M. anisopliae and hosts and the immune mechanism of hosts against pathogens.

Key words: Metarhizium anisopliae, Locusta migratoria manilensis, inhibitor, digestive enzymes, detoxification enzymes

CLC Number:

S476.12

WANG Zhenghao, JIA Miao, ZHANG Yanfeng, DU Guilin, CAO Guangchun, NONG Xiangqun, LIU Changzhong, WANG Guangjun, ZHANG Zehua. Effects of Different Inhibitors on Digestive Enzyme and Detoxification Enzyme in Midgut of Locusta migratoria manilensis during Metarhizium anisopliae Infection[J]. journal1, 2016, 32(6): 756-761.

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