Analysis of Functional Genes of Bacillus thuringiensis Isolates against White Grubs by Comparative Genomics

doi:10.16409/j.cnki.2095-039x.2018.05.007

Abstract

Abstract: In order to discover the key functional genes of insecticidal activity and environmental adaptation from the high toxicity Bacillus thuringiensis (Bt) strains against underground pests, Bt strains 261-1, Fcd114, 1126-1, HBF-18 and 78-2 with high insecticidal activities against Coleoptera were sequenced, as well as lepidoptera-toxic strains WBt-2 and G03. The published genomes of lepidoptera-toxic HD1, HD12, HD73 and Bt185 strains-toxic to white grubs were included in the comparison. Five kind genes and clusters involved in insecticidal, related to the synthesis and degradation of polysaccharide, microbial competition and adaptation, such as aiiA gene (N-Acylhomoserine lactones hydrolase, aiiA), inhA (Immune inhibitor A, inhA) acting on the immune systems of target insects and Zwittermicin A (ZwA), were analyzed and compared respectively. The results indicated that these strains could be divided into three types based on the specific insecticidal genes, they contained:type 1, lepidoptera-specific; type 2, coleoptera-specific; and type 3, both lepidoptera and coleoptera-toxic. Type 2 strains specific to subterranean pests, had specific toxicity-related genes, which were involved in aiiA, and inhA. The results provided a new vision for further improvement of highly effective Bt strains to control underground pests.

Key words: Coleoptera, white grubs, Bacillus thuringiensis, comparative genomics

CLC Number:

S476.1

HUANG Ying, WANG Kui, SHU Changlong, ZHANG Jie. Analysis of Functional Genes of Bacillus thuringiensis Isolates against White Grubs by Comparative Genomics[J]. journal1, 2018, 34(5): 693-700.

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