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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (3): 443-451.DOI: 10.16409/j.cnki.2095-039x.2020.03.029

• SCIENTIFIC NOTES • Previous Articles     Next Articles

Cloning and Bioinformatics Analysis of Voltage-gated Sodium Channel Gene of Myzus persicae

WANG Hao1,2, DUAN Wenbo1,2, LI Fen2, LI Weizheng1, WU Shaoying1,2   

  1. 1. College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, China;
    2. College of Plant Protection, Hainan University, Haikou 570228, China
  • Received:2019-07-15 Online:2020-06-08 Published:2020-06-12

Abstract: The cDNA sequence of the voltage-gated sodium channel gene of Myzus persicae was cloned and the typical characteristics of sodium channel were determined, which laid a foundation for studying the molecular mechanism of resistance to Aphis gossypii. The experimental techniques were mainly RT-PCR and PCR, and the cDNA sequence of the sodium ion channel gene was cloned, and the sequence bioinformatics analysis was carried out by using relevant software. Cloning two cDNA sequences MpNav-1 (NCBI accession number:MN124170) and MpNav-2 (NCBI accession number:MN176136). MpNav-1 is 2945 bp in length and includes a 2877 bp complete open reading frame encoding a total of 958 amino acids. MpNav-2 is 3546 bp in length and includes a 3486 bp complete open reading frame encoding a total of 1161 amino acids. MpNav-1 and MpNav-2 together form the sodium channel of Myzus persicae, MpNav-1 contains a homologous domain I and a homologous domain II MpNav-2 contains a homology domain III and a homologous domain IV. The homology alignment found that the similarity between the Acyrthosiphon pisum and the Melanaphis sacchari sorghum sodium channel was as high as 97.67% and 97.65%, respectively. The cloned sequence contains typical features of the insect sodium ion channel and contains DENS and MFM. Successfully cloned the sodium ion channel gene of Myzus persicae, laying the foundation for elucidating its molecular mechanism of target resistance to pyrethroids.

Key words: Myzus persicae, sodium channel, gene cloning, bioinformatics analysis, phylogenetic analysis

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