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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (4): 558-563.DOI: 10.16409/j.cnki.2095-039x.2020.04.016

• RESEARCH REPORTS • Previous Articles     Next Articles

Effect of Bacillus thuringiensis JQD117 Strain on Intestinal Protease Activity of Larvae of Bradysia odoriphaga

SONG Jian1, CAO Weiping1, GUO Qinggang1, WANG Meng2, LI Shezeng1, DU Lixin1   

  1. 1. Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China;
    2. Wuhan Kernel Biotechnology Co., Ltd, Wuhan 430074, China
  • Received:2019-08-23 Online:2020-08-08 Published:2020-08-07

Abstract: Effect of Bacillus thuringiensis JQD117 strain on activities of protease and detoxification enzymes of Bradysia odoriphaga larvae, including trypsin, chymotrypsin, acetylcholinesterase (AchE), glutathione-S-transferase (GST), and carboxylesterase (CarE), were determined. The LC50 of JQD117 strain to B. odoriphaga 3rd instar larvae at 72 h post treatment was determined to be 2.8070×107cfu/mL by laboratory bioactivity assay. Then 10×LC50, 1×LC50 and 0.1×LC50 concentrations of JQD117 were used to feed and infect the B. odoriphaga 3rd instar larvae. Activities of the five enzymes were measured to the samples regularly collected from the population. The results show that, when the B. odoriphaga 3rd instar larvae were exposed to high concentrations (1×LC50 and 10×LC50) of JQD117, enzyme activities varied relatively widely. Among them, when the B. odoriphaga 3rd instar larvae were infected with 1×LC50 and 10×LC50 concentrations of JQD117, trypsin and AchE activities increased significantly at 24-36 h and 6-60 h compared with the control group, while chymotrypsin and CarE activities were significantly inhibited at 6-60 h compared with the control group. When the B. odoriphaga 3rd instar larvae were infected with 0.1×LC50 concentrations of JQD117, activities of trypsin and AchE were significantly higher than that of the control group only at 36 h and at 12-48 h, respectively; CarE activity was significantly inhibited only at 6 h compared with that of the control group while no significant change in the activity of chymotrypsin was observed. There was no significant change in GST activity at the three infection concentrations compared with the control. Therefore, infection of JQD117 influences on protease and detoxification enzyme activity in B. odoriphaga larvae in a dose-dependent manner. The results provide a theoretical guidance for the application and development of Bt for control of B. odoriphaga.

Key words: Bacillus thuringiensis, Bradysia odoriphaga, protease, detoxifying enzymes

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