Initial Exploration of Cordyceps javanica and Neoseiulus barkeri Combined Control Technology for Tetranychus urticae

doi:10.16409/j.cnki.2095-039x.2024.11.001

Abstract

Abstract: To assess the potential applications of the Cordyceps javanica IJ-tg19 combined with Neoseiulus barkeri for the control of the Tetranychus urticae, the virulence of strain IJ-tg19 against T. urticae and the N. barkeri was determined in indoor experiments. Subsequently, greenhouse and field trials were conducted to evaluate the efficacy of the fungus combined with N. barkeri in controlling T. urticae. The results showed that under the treatment of a spore suspension with a concentration of 1×10⁸ spores/mL, the strain IJ-tg19 had a half-lethal time (LT₅₀) of 3.23 days for the adult stage of the T. urticae, and a lethal concentration (LC₅₀) of 1.67×10⁵spores/mL at 5 days after inoculation. Both biological control measures, whether applied individually or in combination, were effective in controlling the T. urticae, but the combined treatment had a higher control efficacy (96.74%, 98.90%). In the field application trial of “C. javanica-N. barkeri” in the control and treatment areas, there was a significant difference in the population density of the T. urticae. The T. urticae was detected 24 days earlier in the control area compared to the treatment area. The peak population densities in the control area and treatment area were 102 mites/leaf (29, November) and 7.3 mites/leaf (5, December) respectively. This study demonstrates that C. javanica IJ-tg19 combined with N. barkeri is more effective than their individual application, which highlights the potential of the application of fungus and N. barkeri for the prevention and biological control of greenhouse T. urticae.

Key words: Cordyceps javanica, Tetranychus urticae, Neoseiulus barkeri, field trial, biocontrol

CLC Number:

S476.1

ZHAO Yu, MAO Ruixia, XING Peixiang, DIAO Hongliang, LI Xianwei, MA Ruiyan. Initial Exploration of Cordyceps javanica and Neoseiulus barkeri Combined Control Technology for Tetranychus urticae[J]. Chinese Journal of Biological Control, 2024, 40(6): 1250-1259.

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