Colonization of Biocontrol Bacteria YC89 in Different Sugarcane Cultivars and Its Control Effect on Red Rot Disease

doi:10.16409/j.cnki.2095-039x.2024.02.052

Abstract

Abstract: In order to clarify colonization characteristics of Bacillus velezensis YC89 in different sugarcane cultivars and its biocontrol effect against red rot, the present study was carried out to obtain the rifampicinlabeled strain YC89^R, which has stable genetic characteristics and antagonistic activity, by antibiotic labeling method, its colonization characteristics in different sugarcane cultivars were investigated by watering method, and control effect of strain YC89 against red rot was evaluated in four different sugarcane cultivars. Colonization test showed that strain YC89 could colonize in the rhizosphere, roots, stems and leaves of four sugarcane cultivars, and the labeled strain could still be recovered 30 days after inoculation, among which the strongest colonization ability was in the rhizosphere, with the amount of colonization ranging from 8.66× 10⁵ to 7.03× 10⁶ cfu/g, while the least amount of colonization was in leaf, with the content of the strain ranging from 0.72× 10³ to 3.54× 10⁴ cfu/g. The strains had stronger colonization ability in sugarcane cultivars ROC22 and YT93-159. Pot experiment showed that strain YC89 had the best control effect on red rot of ROC22, with the efficacy of 61.11%. The above results indicated that YC89 had high potential application value in the prevention and control of sugarcane red rot

Key words: sugarcane, sugarcane red rot, biocontrol bacteria, colonization, root exudates

CLC Number:

S476

XIE Linyan, HE Lilian, LUO Yanju, LI Yumei, PAI Xianmai, LIU Lufeng, LI Fusheng. Colonization of Biocontrol Bacteria YC89 in Different Sugarcane Cultivars and Its Control Effect on Red Rot Disease[J]. Chinese Journal of Biological Control, 2024, 40(5): 1066-1073.

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