Screening of Bacillus velezensis HMB28023 for Controlling Grape Gray Mold and Identification of Its Antifungal Active Compounds

doi:10.16409/j.cnki.2095-039x.2025.02.005

Abstract

Abstract: Grape gray mold caused by Botrytis cinerea which significantly reduces the yield and quality of grape, but there is a lack of safe and effective microbial fungicides for its control. In this study, the antagonistic activity of 27 bacteria with high antagonistic activity against B. cinerea derived from tomato, was determined by the confrontation culture method. It was found that the inhibition zone and inhibition rate of the same bacterial strain against B. cinerea derived from tomato and grape exhibited variable changes. A bacterial strain HMB28023, was further obtained based on detached leaves method, potting test and field test, which field control efficacy on grape gray mold reached 74.3%. Strain HMB28023 was identified as Bacillus velezensis through morphological characteristics, 16S rDNA as well as phylogenetic tree constructed by using multiple gene sequences of gyrA, gyrB, rpoB and rpoC. The results of detached leaves test indicated that the fermentation broth, cell free supernatant and bacterial suspension of strain HMB28023 all exhibited high biocontrol efficacy against grape gray mold, reaching 81.9%, 75.1% and 64.8%, respectively. The lipopeptide produced by strain HMB28023 was isolated through fast protein liquid chromatography (FPLC) and identified by UPLC-Triple TOF-MS/MS. The results showed that the lipopeptide comprised iturin A (C₁₄—C₁₅), fengycin (fengycin A (C₁₄—C₁₇) and fengycin B (C₁₆—C₁₇)), and surfactin (C₁₂—C₁₆). Both iturin A and fengycin showed strong inhibitory activities against the growth of B. cinerea, and also caused abnormal hyphal growth in vitro.

Key words: Bacillus velezensis, Botrytis cinerea, lipopeptide, biological control

CLC Number:

S476

ZHANG Xiaoyun, WANG Xuemei, CONG Rong, CHEN Xiuye, LU Xiuyun, LI Shezeng, GUO Qinggang, MA Ping. Screening of Bacillus velezensis HMB28023 for Controlling Grape Gray Mold and Identification of Its Antifungal Active Compounds[J]. Chinese Journal of Biological Control, 2025, 41(1): 122-131.

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