Identification and Biological Characteristics of Bacillus velezensis ZY1 against Bacterial Fruit Blotch

doi:10.16409/j.cnki.2095-039x.2024.02.064

Abstract

Abstract: The strain ZY1 was isolated from pepper leaves, which has a significant antagonistic effect on bacterial fruit blotch. Based on morphological observation, Biolog analysis, and multi gene phylogenetic analysis, strain ZY1 was identified as Bacillus velezensis. To evaluate the stress resistance of strain ZY1, its growth status was studied under different pH, NaCl concentration, and temperature. The results showed that strain ZY1 growed rapidly and has strong stress resistance. It could grow normally under conditions of pH 5-9, NaCl concentration of 2%-7%, and 25 ℃-40 ℃, and the growth of the strain was significantly inhibited at 55 ℃. The antibacterial experiment results showed that strain ZY1 had strong antibacterial effects on both group I and group II strain Aacs. The enzymatic test results showed that strain ZY1 could produce metabolites such as cellulase, pectinase, protease, and amylase. At the same time, the strain also had the ability to hydrolyze phosphorus and produce ammonia. This article reported for the first time a strain of Bacillus velezensis, which had antibacterial activity against bacterial fruit blotch. This strain ZY1 provided a new microbial resource for the biological control and soil improvement.

Key words: Bacillus velezensis, identification, stress resistance, biological control

CLC Number:

S476

YANG Yuwen, LIU Dehua, WANG Chengliang, MENG Yonghong, GUAN Wei, ZHAO Tingchang. Identification and Biological Characteristics of Bacillus velezensis ZY1 against Bacterial Fruit Blotch[J]. Chinese Journal of Biological Control, 2024, 40(6): 1430-1438.

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