Screening, Identification and Determination of Antagonistic Actinomycetes Strain against Tea Anthracnose

doi:10.16409/j.cnki.2095-039x.2023.02.031

Abstract

Abstract: Tea anthracnose is an important foliar disease of tea plants, which seriously affects the yield and quality of tea when it occurs and brings economic losses to tea production. In order to obtain actinomycetes with good antagonistic effect against tea anthracnose caused by Colletotrichum camelliae, dilution coated plate method was used to isolate the actinomycetes from the rhizosphere soil of tea plantations, and strains with high antagonistic activity were screened using the plate confrontation method; their morphological characteristics, culture characteristics, physiological and biochemical properties and molecular biology were combined to identify their species. The growth inhibition of JS4-F against tea anthracnose was observed by scanning electron microscopy, the indoor control effect of its fermentation solution against tea anthracnose was determined by in vitro leaf method and pot experiment, and its antibacterial spectrum was determined by plate standoff method. The results showed that strain JS4-F with good antagonistic effect against tea anthracnose was isolated and screened, and it was initially identified as Streptomyces spp. based on its morphological characteristics and physiological and biochemical properties, and a phylogenetic tree was constructed by combining the sequencing results of 16SrRNA, gyrB,ropB, recA and trpB genes, and the strain JS4-F was identified as Streptomyces luteospore. The antagonistic effect of strain JS4-F against tea anthracnose reached 75.59%, and scanning electron microscopy showed that strain JS4-F could deform, wrinkle and intertwine the mycelium of tea anthracnose. The nitrogen source utilization test showed that strain JS4-F could utilize L-arginine, L-glycine, L-alanine, and L-tryptophan, could grow at 6% NaCl concentration and pH tolerance range was 5-12. The control effect of strain JS4-F fermentation broth was 52.54% on isolated leaves indoors, 39.94% after 10 times dilution and 30.56% after 100 times dilution. The pot control test showed that the control effect of strain JS4-F fermentation broth on tea anthracnose was 44.59%. The antagonistic effect of strain JS4-F on the other 8 plant pathogens is greater than 60%. In conclusion, strain JS4-F has a good potential for the control of tea anthracnose, and can provide reference resources and technical basis for the biological control of tea anthracnose.

Key words: tea anthracnose, Streptomyces, antagonistic effect, biocontrol

CLC Number:

S476

ZHANG Yudan, TAN Lin, REN Zuohua, YANG Yishuai, YANG Xueyu, HU Qiulong. Screening, Identification and Determination of Antagonistic Actinomycetes Strain against Tea Anthracnose[J]. Chinese Journal of Biological Control, 2023, 39(3): 646-656.

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