Discovery of Key Genes and Pathway Mechanisms Driving Efficient Gougerotin Biosynthesis

doi:10.16409/j.cnki.2095-039x.2026.02.037

Abstract

Abstract: This study aimed to identify key regulatory elements in the gougerotin biosynthetic gene cluster(BGC) by constructing strains overexpressing the gouA-gouL genes in Streptomyces noourise CK-15. Through comprehensive phenotypic analysis, metabolite detection, and proteomics, the overexpression strains were systematically investigated for their growth, sporulation, antimicrobial activity, gougerotin production, and regulatory effects on the metabolic network. The results demonstrated that strains overexpressing gouC(encoding an acetyltransferase) and gouD(encoding a methyltransferase) exhibited earlier sporulation compared to the wild-type,4 days after cultivation. Mycelial growth rates were significantly higher during the 0–120 h period, and antimicrobial activity against Rhodotorula increased by 37.34% and 24.33%, respectively. Compared to the wild-type, gougerotin production in the gouC and gouD overexpressing strains increased by 194.82% and 163.28%, reaching 2.23 g/L and 1.99 g/L, respectively. Proteomic analysis further revealed that overexpression of these two genes activated carbohydrates and amino acids metabolism pathways and enhanced the supply of UDP-glucuronic acid precursors. This study extends our knowledge of the regulatory mechanisms in gougerotin biosynthesis and provides theoretical and scientific foundations for constructing high-yield strains and optimizing their metabolic networks.

Key words: Streptomyces, gougerotin, proteomics, high-yield modification

CLC Number:

S476

YU Hua, ZHOU Qianying, LIAO Yutong, ZANG Zhengyi, GE Beibei. Discovery of Key Genes and Pathway Mechanisms Driving Efficient Gougerotin Biosynthesis[J]. Chinese Journal of Biological Control, 2026, 42(4): 886-897.

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