Screening of Antagonistic Streptomyces 26B and Its Efficacy on the control of Soft Rot Disease of Chinese Cabbage in Soil with Different Water Content

doi:10.16409/j.cnki.2095-039x.2022.02.034

Abstract

Abstract: The purpose of this study is to obtain the biocontrol bacteria to control the soft rot disease of Chinese cabbage. An antagonistic strain 26B was screened from 38 actinomycete strains isolated from the rhizosphere of Chinese cabbage by Oxford cup method. Strain 26B was identified as Streptomyces based on its morphological characteristics and its 16S rRNA gene sequence analyses. The cell free fermentation filtrate of strain 26B showed high antibacterial activity against Pectobacterium carotovorum subsp. brasiliensis BC1 and three casual bacteria of potato blackleg disease, and the antibacterial diameter against BC1 reached 23.97 mm. The germination assay by seed-soaking showed that the cell free fermentation filtrate of strain 26B significantly promoted the germination potential, germination index, root length and fresh weight of Chinese cabbage seeds. Effects of cell free fermentation filtrate of strain 26B on the inhibition of BC1-gfp colonized on the Chinese cabbage roots was examined by root-dipping method. The results showed that the number of BC1-gfp on cabbage roots reached 2.3×10⁹ CFU/g after 48 hours’ incubation in the treatment with BC1-gfp suspension, while no BC1-gfp was detected on the cabbage roots treated with the premixed mixture of BC1-gfp suspension and sterilized fermentation filtrate of strain 26B at a volume ratio of 10%. The bioassay test in potting soil showed that the efficacy of cell free fermentation filtrate of strain 26B on the control of soft rot disease reached 96.0% and 89.8%, respectively, in soil with water content of 14% and 20%. The above results indicated that Streptomyces 26B has great potential to be applied as a biocontrol strain, and its biocontrol efficacy can be improved through coordinately management of soil water content.

Key words: soft rot pathogen of Chinese cabbage, Streptomyces, 16S rRNA gene, antibacterial activity, biological control

CLC Number:

S476

ZHANG Shichang, ZHANG Yulu, TAN Xiaodong, GUO Rongjun, LI Shidong, LUO Ming. Screening of Antagonistic Streptomyces 26B and Its Efficacy on the control of Soft Rot Disease of Chinese Cabbage in Soil with Different Water Content[J]. Chinese Journal of Biological Control, 2023, 39(1): 157-166.

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