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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (1): 250-257.DOI: 10.16409/j.cnki.2095-039x.2021.08.007

• RESEARCH REPORTS • Previous Articles     Next Articles

Stability of Cultural Filtrate and Crude Extract Produced by Streptomyces sp. 3-10

LÜ Ang1,2, WU Mingde3, ZHANG Jing3, YANG Long3, LI Guoqing3   

  1. 1. Institute of Quality Standard and Testing Technology for Agro-products, Hubei Academy of Agricultural Science, Wuhan 430064, China;
    2. Hubei Key Laboratory of Nutritional Quality and Safety of Agro-products, Wuhan 430064, China;
    3. College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2021-07-19 Online:2022-02-08 Published:2022-03-01

Abstract: In order to better utilize the secondary metabolites from Streptomyces sp. 3-10, the crude extracts with antifungal activity were extracted by using different organic solvents from the cultural filtrate of Streptomyces sp. 3-10. The stability of the antifungal substance was investigated for response to temperature, pH, UV-irradiation and storage conditions. The results showed that ethyl acetate was the best organic solvent to extract the antifungal substance from the cultural filtrate, as the crude extract by ethyl acetate exhibited high antifungal activity against Botrytis cinerea. The crude extract was sensitive to temperature higher than 80℃ UV-irradiation for 1 min could compromise the antifungal activity of the crude extract, and the antifungal activity of the crude extract nearly lost after UV-irradiation for 5 min. The crude extract showed high antifungal activity in acidic condition, but lost in alkali condition. The lyophilized powder of the crude extract could maintain its antifungal activity at 4℃ and room temperature for 100 d. The stability and storage condition study of the crude extract can provide data support for future applying of Streptomyces sp. 3-10 as a biocontrol agent.

Key words: Streptomyces sp. 3-10, crude extract, stability, storage condition, antifungal activity

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