Stability of Cultural Filtrate and Crude Extract Produced by Streptomyces sp. 3-10

doi:10.16409/j.cnki.2095-039x.2021.08.007

Abstract

Abstract: In order to better utilize the secondary metabolites from Streptomyces sp. 3-10, the crude extracts with antifungal activity were extracted by using different organic solvents from the cultural filtrate of Streptomyces sp. 3-10. The stability of the antifungal substance was investigated for response to temperature, pH, UV-irradiation and storage conditions. The results showed that ethyl acetate was the best organic solvent to extract the antifungal substance from the cultural filtrate, as the crude extract by ethyl acetate exhibited high antifungal activity against Botrytis cinerea. The crude extract was sensitive to temperature higher than 80℃ UV-irradiation for 1 min could compromise the antifungal activity of the crude extract, and the antifungal activity of the crude extract nearly lost after UV-irradiation for 5 min. The crude extract showed high antifungal activity in acidic condition, but lost in alkali condition. The lyophilized powder of the crude extract could maintain its antifungal activity at 4℃ and room temperature for 100 d. The stability and storage condition study of the crude extract can provide data support for future applying of Streptomyces sp. 3-10 as a biocontrol agent.

Key words: Streptomyces sp. 3-10, crude extract, stability, storage condition, antifungal activity

CLC Number:

S476

LÜ Ang, WU Mingde, ZHANG Jing, YANG Long, LI Guoqing. Stability of Cultural Filtrate and Crude Extract Produced by Streptomyces sp. 3-10[J]. Chinese Journal of Biological Control, 2022, 38(1): 250-257.

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