一株分离自德国小蠊的贵州绿僵菌的鉴定及生物学特性

doi:10.16409/j.cnki.2095-039x.2021.07.021

摘要/Abstract

摘要： 从安徽省宣城市麻姑山林场采集到自然罹病的德国小蠊僵虫，通过分离纯化获得一株绿僵菌RCEF6416，采用显微形态和分子生物学相结合方法将该菌株鉴定为贵州绿僵菌。本试验研究了在不同培养基、盐浓度、pH以及高温胁迫等条件下，该菌株的培养特征及产孢能力，以期为世界性卫生害虫德国小蠊的生物防治提供种质资源和理论依据。结果表明：该菌株在PPDA培养基上的生长速度较快，菌落直径平均日增量为4.94 mm/d，但在PDA培养基上产孢量最大，为5.1×10⁷孢子/cm²；在一定范围内，随着盐浓度增加菌落直径日增量增加，但是产孢量逐渐下降，且菌落逐渐产生白色菌丝圈；当pH为8时，菌株生长速度最快，产孢量最大；高温对菌株孢子萌发率影响很大，当水浴温度为45℃处理4 h后，其孢子萌发率仅为2.6%。

关键词: 贵州绿僵菌, 蟑螂, 生物防治, 培养条件

Abstract: In this study, a Metarhizium strain RCEF6416 was isolated from naturally diseased Blattaria germanica adults collected from Magu mountains forestry center, Xuancheng, Anhui Province. The pathogen was identified as Metarhizium guizhouense by morphological and molecular biological methods. To provide strain resource and theoretical basis for biological control of the important health pest B. germanica, we investigated the culture growth and sporulation ability under different conditions of culture medium, salt concentration, pH value, and high temperature stress. The results showed that the strain RCEF6416 grew the fastest on PPDA medium, with daily increase of colony diameter of 4.94 mm/d. However, the strain sporulated the highest on PDA medium, reaching 5.1×10⁷ spores/cm². In a certain range of salt concentration, the daily increase of colony diameter increased with the increase of salt concentration, whereas the spore production decreased gradually, and the colony produced white hyphae circle. The optimum pH for culture growth and sporulation capacity was 8. High temperature inhibited the spore germination rate. When treated for 4 h at water bath of 45℃, the spore germinated at only 2.6%.

Key words: Metarhizium guizhouense, cockroach, biological control, culture conditions

中图分类号:

S476.1

陈名君, 汪婷, 卞林猛, 林俨, 黄勃. 一株分离自德国小蠊的贵州绿僵菌的鉴定及生物学特性[J]. 中国生物防治学报, 2021, 37(6): 1344-1352.

CHEN Mingjun, WANG Ting, BIAN Linmeng, LIN Yan, HUANG Bo. Identification and Biological Characteristics of a Metarhizium guizhouense Strain Isolated from Blattaria germanica[J]. Chinese Journal of Biological Control, 2021, 37(6): 1344-1352.

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