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journal1 ›› 2017, Vol. 33 ›› Issue (6): 817-825.DOI: 10.16409/j.cnki.2095-039x.2017.06.015

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Bio-control of PGPR Seed Coating Producing ACC Deaminase to Cucumber Bacterial Stem Soft Rot Disease

HE Zidian1, YAN Liying1, SHI Yanxia2, LI Baoju2, GAO Yufeng1, ZHANG Mingzhu1, SUN Jian1   

  1. 1. Hebei Normal University of Science and Technology, Qinhuangdao 066004, China;
    2. Institute of Vegetable and Flower, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2017-09-08 Online:2017-12-08 Published:2017-12-16

Abstract: The plant growth promoting rhizo bacteria (PGPR) producing 1-amino cyclopro-pane-1-carboxylic acid (ACC) deaminase were isolated and identified from the rhizosphere soil of seashore halophytesin order to prevent and control cucumber bacterial stem soft rot, a new disease in winter greenhouse cucumber in recent years. 7 species of PGPRincluding Stenotrophomonas maltophilia, Raoultella terrigena, Agrobacterium tumefaciens, Klebsiella oxytoca,Pantoea agglomerans,Enterobacter aerogenes and Kluyvera cryocrescens were identified. The activity of ACC deaminase in Stenotrophomonas maltophilia CRG-2 was the highest, 324.3 μmol/(mg·h) in 22 strains. At the same time, the biocontrol effect of biological seed-coating by mixing isolate strain CRG-2 and Bacillus subtilis with additives to cucumber bacterial stem soft rot were tested in the field. The control effect of the seed coating agent formed by the mixture of strain CRG-2and Bacillus subtilis to cucumber bacterial stem soft rot reached 73.13% in the field, higher than zhongshengmycin with control effect of 15.79%.The encouraging effect of seeding coat on biological control suggested that it had the potential to be applied widely in the cucumber bacterial stem soft rot control in the future.

Key words: plant growth promoting rhizobacteria (PGPR), Stenotrophomonas maltophilia, ACC deaminase, cucumber bacterial stem soft rot, control effect

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