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Chinese Journal Of Biological Control ›› 2019, Vol. 35 ›› Issue (4): 605-612.DOI: 10.16409/j.cnki.2095-039x.2019.04.018

• RESEARCH REPORTS • Previous Articles     Next Articles

Differential Analysis between Phytophthora nicotianae from Tobacco and Its Antagonistic Bacterial Bacillus amyloliquefaciens in Metabolic Phenotypic Characterization

ZHAO Xiaochao1, CHEN Qianli2,5, YANG Shuangjian4, LIU Chang3,5, WANG Hancheng5   

  1. 1. Hubei Zhongyan Industry Co., Ltd., Wuhan 430040, China;
    2. College of Agriculture, Guizhou University, Guiyang 550025, China;
    3. College of Life Sciences, Yangtze University, Jingzhou 434025, China;
    4. Guizhou Tongren Tobacco Company, Tongren 554300, China;
    5. Guizhou Academy of Tobacco Science, Guiyang 550081, China
  • Received:2018-11-22 Online:2019-08-08 Published:2019-08-10

Abstract: Phytophthora nicotianae is the phytopathogen that causes tobacco black shank. An antagonistic bacterial isolate Bacillus amyloliquefaciens was found in our early study, which presented high inhibition effect on P. nicotianae. In order to assess the potential application of this antagonistic bacterium, the differential analysis of metabolic phenotype between the B. amyloliquefaciens and P. nicotianae were conducted in this study. Results presented that the metabolic fingerprint of these two microorganisms were quite different. P. nicotianae and B. amyloliquefaciens metabolized 74% and 41% of the tested carbon substrates, 96% and 77% of the tested nitrogen substrates, 98% and 86% of the tested phosphorus substrates, 100% and 69% of the tested sulfur substrates, respectively. They had 94 and 91 different biosynthetic pathways, metabolized 72 and 95 different osmolyte, and could metabolize in 95 and 94 different pH environments, respectively. P. nicotianae showed stronger ability than B. amyloliquefaciens in the metabolism numbers of carbon, nitrogen, phosphorus and sulfur substrates, while poorer ability than B. amyloliquefaciens in osmolyte environments. The typical carbon that B. amyloliquefaciens efficiently utilized while not for P. nicotiana was D-glucosamine. They presented nearly equal metabolic abilities in both biosynthetic pathways and pH environments. They both had decarboxylase activities and deaminase activities. These results provided some valuable foundation for developing the bio-control agents of B. amyloliquefaciens against tobacco black shank.

Key words: Bacillus amyloliquefaciens, Phytophthora nicotianae, metabolic phenotypic characterization

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