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journal1 ›› 2019, Vol. 35 ›› Issue (3): 399-406.DOI: 10.16409/j.cnki.2095-039x.2019.03.011

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Growth-promotion Effect of Bacillus amyloliquefaciens PHODB35 on Wheat Plant and Yield

ZHAO Weisong1, LI Shezeng1, LU Xiuyun1, ZHANG Xiaoyun1, WANG Peipei1, GUO Ginggang1, QI Yongzhi2, DONG Lihong1, MAO Xiaoxi1, SU Zhenhe1, MA Ping1   

  1. 1. Integrated Pest Management Center of Hebei Province/Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, China;
    2. College of Plant Protection, Agricultural University of Hebei, Baoding 071000, China
  • Received:2018-07-18 Online:2019-06-08 Published:2019-06-17

Abstract: Bacillus amyloliquefaciens PHODB35 is a multifunctional bacterial strain which has significant control efficiency on diseases and growth-promotion effect on plants. In order to clarify the growth promoting effect of strain PHODB35 on wheat, seed germination and seedling growth treated with fermentation broth of strain PHODB35 were investigated by petri dish method in vitro. The results showed that the fermentation broth of strain PHODB35 had different effects on seed germination and bud length, and the high concentration of fermentation broth at 10×108 and 2×108CFU/mL had inhibitory effects,, respectively. Moreover, there was no significant difference on germination rate at the low concentration of 1×108, 1×107, 2×106 and 1×106 CFU/mL, but promoted the growth of bud growth, by 13.48% to 20.22%, respectively. It was preliminarily cleared that the suitable inoculum concentration of strain PHODB35 was 1×108 CFU/mL. Pot experiment in greenhouse showed that the plant height, fresh weight, dry weight of wheat and available phosphorus content in substrate was increased to some extent under the suitable inoculum concentration of strain PHODB35. At the same time, the organic fertilizer (concentration of 1×108 CFU/g) was developed with the active component of strain PHODB35. Pot experiments showed that the content of available phosphorus in substrate and total phosphorus in wheat were increased by 3.86 times and 3.55 times, respectively, with the increase of application of PHODB35 organic fertilizer. It was concluded that the growth-promoting effect of PHODB35 was better than that of common organic fertilizer. Field experiments in many areas showed that the yield of PHODB35 organic fertilizer (T3) was higher than that of chemical fertilizer treatment (T2), with the increase rate of 5.31% to 9.06%. The results provided a scientific basis for further industrial development and application of Bacillus amyloliquefaciens PHODB35 organic fertilizer.

Key words: Bacillus amyloliquefaciens, growth-promoting effects, wheat, chemical fertilizer reduction, organic fertilizer

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