Efficiency and Mechanism of Bio-organic Fertilizer in Suppressing Wheat Take-all Pathogen Gaeumannomyces graminis var. tritici

doi:10.16409/j.cnki.2095-039x.2016.01.017

Abstract

Abstract: The pot trials, dual culture in Petri dish, physiological and biochemical analysis were used to investigate the efficiency and mechanism of bio-organic fertilizer against take-all disease of wheat. The incidence of the disease was significantly decreased by 53.44% in pots amended with the bio-organic fertilizer. Furthermore, application of the fertilizer significantly increased root number and tiller number of wheat. Chlorophyll content increased by 1.08 times and 1.15 times, root number increased by 1.25 and 1.22 times, root activity increased by 1.2 and 1.4 times compared to control after leaf spraying and root soaking with the fertilizer, respectively. Crude extracts of the bioorganic fertilizer inhibited mycelium growth of Gaeumannomyces graminis var. tritici in Petri dish and its active substance showed heat stable. A Bacillus strain was isolated from the bio-organic fertilizer. This strain effectively suppressed the mycelial growth of the pathogen. Soil analysis indicated that the content of organic matter, available potassium, organic phosphorus and hydrolysable nitrogen in soil were increased by 12.6%, 16.3%, 52.1% and 6.9%, respectively, compared to the control. Defense-related enzymes of PAL, POD and PPO also increased compared to control plants. These results demonstrate that the bio-organic fertilizer is capable of protecting wheat from Gaeumannomyces graminis var. tritici through a variety of mechanisms.

Key words: Gaeumannomyces graminis var. tritici, bioorganic fertilizer, control efficiency, mechanism

CUI Shichun, YANG Xiufen, ZHENG Xingyun, YANG Huaiwen. Efficiency and Mechanism of Bio-organic Fertilizer in Suppressing Wheat Take-all Pathogen Gaeumannomyces graminis var. tritici[J]. journal1, 2016, 32(1): 112-118.

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