Pathogenicity and Extracellular Enzyme Activities of Metarhizium anisopliae to Ectropis obliqua hypulina Wehrli

doi:10.16409/j.cnki.2095-039x.2021.01.023

Abstract

Abstract: Fungal strains were identified using morphological characteristics and rDNA-ITS sequence from entomogenous fungi isolated from insect cadaver in the humic layer of tea garden and evaluated for their biocontrol potential and extracellular enzyme activities against the 4th instar larvae of Ectropis obliquus. The isolated fungus was identified as Metarhizium anisopliae CHMA-005. After infection of the 4^th instar larvae of E. obliquus for 10 days, the strain CHMA-005 resulted in mortality, LC₅₀ and LT₅₀ of 100%, 1.84×10⁴ cfu/mL and 3.57 d, respectively. Protease activity and chitinase activity peaked on the 6^th day after infection, which were 31.43 U/mL and 27.73 U/mL, respectively. Therefore, with high pathogenicity and extracellular enzyme activities to E. obliqua, M. anisopliae strain CHMA-005 has good biocontrol potential and application prospect.

Key words: Metarhizium anisopliae, toxicity, extracellular enzyme activity, Ectropis oblique

CLC Number:

S476.1

CHEN Feng, WANG Changfang, WANG Jun, CHEN Wenle, HU Jinfeng, WU Wei, YAO Jinai. Pathogenicity and Extracellular Enzyme Activities of Metarhizium anisopliae to Ectropis obliqua hypulina Wehrli[J]. Chinese Journal of Biological Control, 2021, 37(1): 124-129.

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