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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (1): 188-195.DOI: 10.16409/j.cnki.2095-039x.2021.03.024

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Tolerance of Different Entomopathogenic Nematodes to Environmental Stresses

MA Juan, Guo Xiaoxiao, LI Xiuhua, WANG Rongyan, GAO Bo, CHEN Shulong   

  1. Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, Baoding 071000, China
  • Received:2020-10-10 Online:2022-02-08 Published:2022-03-01

Abstract: As an efficient biological control agent, entomopathogenic nematode (EPN) has been increasingly used in the pest control. However, entomopathogenic nematode is greatly affected by natural conditions. Therefore, it is of great significance to screen stress resistant nematode strains for local pest control. The interspecific variation in stress resistance and biocontrol potential were compared among 17 EPN strains in vitro. Steinernema tielingense LFS65, S. ceratophorum HQA87, and S. carpocapsae All showed higher heat tolerance than the other strains, with mortalities less than 10% after exposure to 40℃ for 6 h. High cold tolerance was observed in Steinernema sp ZLS3 and S. carpocapsae All, with mortality of 1.14% and 1.93% after exposure to -5℃ for 24 h, respectively; followed by S. feltiae LFS67, S. feltiae JY90, and H. beicherriana NCWZ1. Steinernema sp ZLS3, S. carpocapsae All, and S. ceratophorum HQA87 exhibited higher level of desiccation tolerance than the others, over 90% of the nematodes survived exposure to 25% glycerol for 72 h. The infectivity of the 9 selected EPN strains to Galleria mellonella was determined. Mortality of G. mellonella larvae increased to 100% after exposure to IJs of S. ceratophorum HQA87, S. tielingense LFS65, S. feltiae JY-90, and S. carpocapsae all for 36 h. The results indicate that these EPN may have great potential for use in integrated pest management.

Key words: entomopathogenic nematode, biological control, tolerance, infectivity

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