Screening and Identification of Biocontrol Strain Claroideoglomus etunicatum N62 against Ginger Wilt

doi:10.16409/j.cnki.2095-039x.2022.02.002

Abstract

Abstract: Ginger wilt is a devastating disease caused by the soil-borne bacterial Ralstonia solanacearum. Till now, there is still no bactericides available for effective control of the disease in the field. Arbuscular mycorrhizal fungi have great potential in promoting crop growth and preventing diseases. In order to explore the biocontrol agents against ginger wilt, four Arbuscular mycorrhizal fungi strains were selected to evaluate the ginger growth promotion ability and control efficacy against the ginger wilt through pot culture method, and the dominant strain N62 was identified based on morphological characteristics and 18S rDNA sequence analysis. All the four arbuscular mycorrhizal fungi strains had the ability of promoting ginger growth after inoculation. The control efficacy of N62 against the ginger wilt was found up to 58.6% in the pot culture method, significantly higher than that of other arbuscular mycorrhizal fungi strains. Morphological observation and 18S rDNA sequence alignment analysis results showed that the N62 strain belonged to Claroideoglomus etunicatum. These results indicated that the strain N62 was of development and utilization value for biological control of ginger wilt disease.

Key words: ginger wilt, arbuscular mycorrhizal fungi, Claroideoglomus etunicatum, biological control

CLC Number:

S476

WANG Qian, LI Dongping, QIN Xiaojuan, CHE Jianglü, SONG Juan, CHEN Tingsu. Screening and Identification of Biocontrol Strain Claroideoglomus etunicatum N62 against Ginger Wilt[J]. Chinese Journal of Biological Control, 2022, 38(1): 267-274.

References

[1] 王胜利, 龚晓, 周双德, 等. 姜瘟致病菌的分离与鉴定及其拮抗菌的筛选[J]. 湖南农业大学学报(自然科学版), 2013, 39(3):282-285.
[2] 张瑞华, 刘振龙, 王承香, 等. 不同时期用药对姜瘟病的防治效果[J]. 现代农业科技, 2021(4):94-103.
[3] Zhang X Y, Zhang H J, Zhang Y X, et al. Arbuscular mycorrhizal fungi alter carbohydrate distribution and amino acid accumulation in Medicago truncatula under lead stress[J]. Environmental and Experimental Botany, 2020, 171:103950.
[4] 张哲超, 杨久扬, 郝百惠, 等. 微生物群落驱动AM真菌、生物炭及联合改良沙化土壤作用潜力[J]. 环境科学, 2021, 42(4):2066-2078.
[5] Matthias C R, Noor F M, Evaf L, et al. Mycelium of arbuscular mycorrhizal fungi increases soil water repellency and is sufficient to maintain water-stable soil aggregates[J]. Soil Biology and Biochemistry, 2010, 42(7):1189-1191.
[6] Naheeda B, Muhammad A A, Zhang L X. AMF inoculation and phosphorus supplementation alleviates drought induced growth and photosynthetic decline in Nicotiana tabacum by up-regulating antioxidant metabolism and osmolyte accumulation[J]. Environmental and Experimental Botany, 2020, 176:104088.
[7] Crossay T, Cavaloc Y, Majorel C, et al. Combinations of different arbuscular mycorrhizal fungi improve fitness and metal tolerance of sorghum in ultramafic soil[J]. Rhizosphere, 2020, 14:100204.
[8] 晋治波, 解玲, 朱正杰. 丛枝菌根真菌对不同番茄品种抗根结线虫病的影响[J]. 微生物学通报, 2021, 48(3):755-764.
[9] 李应德, 闰智臣, 高萍, 等. 摩西球囊霉对紫花苜蓿烟色织孢霉根腐病的影响[J]. 中国生物防治学报, 2018, 34(4):598-605.
[10] 王维华, 李敏, 刘润进, 等. AM真菌对生姜某些生理指标的影响[J]. 莱阳农学院学报, 2003, 20(3):175-177.
[11] 刘贵猛, 谭树朋, 孙文献, 等. AMF和PGPR对生姜青枯病的影响[J]. 菌物学报, 2017, 15(1):1-7.
[12] 汪茜, 龙艳艳, 李冬萍, 等. 5种染色剂对生姜根系丛枝菌根(AM)真菌的染色效果比较[J]. 南方农业学报, 2015, 46(8):1425-1429.
[13] Liu X D, Feng Z W, Zhao Z Y, et al. Acidic soil inhibits the functionality of arbuscular mycorrhizal fungi by reducing arbuscule formation in tomato roots[J]. Soil Science and Plant Nutrition, 2020, 66(2):275-284.
[14] 盛萍萍, 刘润进, 李敏. 丛枝菌根观察与侵染率测定方法的比较[J]. 菌物学报, 2011, 30(4):519-525.
[15] 农药田间药效试验准则. 第31部分:杀菌剂防治生姜姜瘟病[S]. 标准号:NY/T 1464.31-2010.
[16] 王幼珊, 刘润进. 球囊菌门丛枝菌根真菌最新分类系统菌种名录[J]. 菌物学报, 2017, 36(7):820-850.
[17] 张金莲, 刘金华, 包涵, 等. 丛枝菌根真菌与胡萝卜毛状根双重培养体系研究[J]. 热带作物学报, 2020, 41(8):1535-1542.
[18] 董秀丽, 赵斌. 嵌套多重PCR-研究田间植物部分丛枝菌根真菌和微生物区系的一个可行技术[J]. 中国科学, 2006, 36(1):59-65.
[19] 叶文兰, 骆礼华, 严璐, 等. 金佛山方竹根际土壤中优势AM真菌的鉴定[J]. 竹子学报, 2017, 36(2):36-43.
[20] Bora P, Bora L C, Deka P C, et al. Efficacy of Pseudomonas fluorescens and Trichoderma viride based bioformulation for management of bacterial wilt disease of ginger[J]. International Journal of Plant Sciences, 2016, 11(2):180-186.
[21] 杨合同, 唐文华. 植病生防菌株B1301的种类鉴定及其对生姜青枯病的作用机理和防治效果[J]. 中国生物防治, 2002, 18(1):21-23.
[22] 李霞, 张小平, 李欣. 一株拮抗姜瘟根际青枯假单胞杆菌的沙雷氏菌的分离与鉴定[J]. 湖北农业科学, 2014, 53(20):4841-4844.
[23] Ram P, Mathur K, Ram J. Response of application methods of biocontrol agents either as rhizome pelleting or as soil application, or as both against rhizome rot of ginger[J]. Annals of Biology, 1997(2):293-296.
[24] Gosling P, Hodge A, Goodlass G, et al. Arbuscular mycorrhizal fungi and organic farming[J]. Agriculture Ecosystems and Environment, 2006, 113(14):17-35.
[25] 陈保冬, 于萌, 郝志鹏, 等. 丛枝菌根真菌应用技术研究进展[J]. 应用生态学报, 2019, 30(3):1035-1046.
[26] 王倡宪, 郝志鹏. 丛枝菌根真菌对黄瓜枯萎病的影响[J]. 菌物学报, 2008, 27(3):395-404.
[27] 刘茂军, 张兴涛, 赵之伟. 深色有隔内生真菌(DSE)研究进展[J]. 菌物学报, 2009, 28(6):888-894.
[28] Wu L Q, Lv Y L, Meng Z X, et al. The promoting role of an isolate of dark-septate fungus on its host plant Saussurea involucrata Kar. et Kir[J]. Mycorrhiza, 2010, 20:127-135.