Screening and Control Effect of Rare Antagonistic Actinomycetes against Rice Blast

doi:10.16409/j.cnki.2095-039x.2021.02.015

Abstract

Abstract: To screen the rare actinomycetes with good antifungal effect against the Magnaporthe oryzae. Actinomycetes were isolated from rhizosphere soil of Taxus chinensis. The results showed that a total of 11 strains of actinomycetes were isolated. Strain ST7−2 showed the best inhibitory effect on M. oryzae, and the inhibitory rate was 79.88%. ST7−2 was identified as Streptomyces tubercidicus combining 16S rRNA, recA, gyrB, rpoB sequence analysis, and the morphological, physiological, and biochemical characteristics. In addition, ST7−2 displayed good effects on Magnaporthe oryzae, Colletotrichum truncatum, Phomopsis asparagi, Ustilaginoidea virens and Mycogone perniciosa. The control effect of fermentation broth against seeding blast was 82.76% under the greenhouse, which showed no significant difference with that of 75% tricyclazole wettable powder 1700 fold. In field trials, the control effect of fermentation broth was 73.57%, which was slightly less effective than 75% tricyclazole WP. Therefore, S. tubercidicus ST7−2 is a potential biocontrol strain against rice blast, and show a wild prospect for development and application in the future.

Key words: rare actinomycetes, rice, Magnaporthe oryzae, antifungal effect, taxonomic identification

CLC Number:

S435.11
S476

RUAN Hongchun, SHI Niuniu, DU Yixin, CHEN Furu. Screening and Control Effect of Rare Antagonistic Actinomycetes against Rice Blast[J]. Chinese Journal of Biological Control, 2021, 37(3): 538-546.

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