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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (3): 538-546.DOI: 10.16409/j.cnki.2095-039x.2021.02.015

• RESEARCH REPORTS • Previous Articles    

Screening and Control Effect of Rare Antagonistic Actinomycetes against Rice Blast

RUAN Hongchun, SHI Niuniu, DU Yixin, CHEN Furu   

  1. Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
  • Received:2020-11-03 Published:2021-03-30

Abstract: To screen the rare actinomycetes with good antifungal effect against the Magnaporthe oryzae. Actinomycetes were isolated from rhizosphere soil of Taxus chinensis. The results showed that a total of 11 strains of actinomycetes were isolated. Strain ST7−2 showed the best inhibitory effect on M. oryzae, and the inhibitory rate was 79.88%. ST7−2 was identified as Streptomyces tubercidicus combining 16S rRNA, recA, gyrB, rpoB sequence analysis, and the morphological, physiological, and biochemical characteristics. In addition, ST7−2 displayed good effects on Magnaporthe oryzae, Colletotrichum truncatum, Phomopsis asparagi, Ustilaginoidea virens and Mycogone perniciosa. The control effect of fermentation broth against seeding blast was 82.76% under the greenhouse, which showed no significant difference with that of 75% tricyclazole wettable powder 1700 fold. In field trials, the control effect of fermentation broth was 73.57%, which was slightly less effective than 75% tricyclazole WP. Therefore, S. tubercidicus ST7−2 is a potential biocontrol strain against rice blast, and show a wild prospect for development and application in the future.

Key words: rare actinomycetes, rice, Magnaporthe oryzae, antifungal effect, taxonomic identification

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