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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (5): 1024-1034.DOI: 10.16409/j.cnki.2095-039x.2021.06.006

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Inhibition Effect of Antimicrobial Peptide Isolated from Bacillus methylotrophicus G-1 against Botrytis cinerea

ZHANG Baojun, MA Chao, GUO Jiexin, SUN Zhuonan, ZHANG Xiaojie   

  1. College of Plant Protection, Shanxi Agricultural University, Taigu 030801, China
  • Received:2021-01-06 Online:2021-10-08 Published:2021-10-28

Abstract: This study aims to explore the mechanism of inhibitory effect of Bacillus methylotrophicus G-1 on Botrytis cinerea. High-performance liquid chromatography and liquid chromatography-mass spectrometry were used to isolate and identify the antimicrobial peptides produced by strain G-1. Scanning electron microscopy was performed to observe the influence of the antimicrobial peptides on the hyphal morphology of B. cinerea. RNA-seq was used to investigate the differentially expressed genes after antimicrobial peptide treatment for 3 and 5 days. The results showed that the main antimicrobial peptide produced by B. methylotrophicus G-1 was iturin A. After being treated with the antimicrobial peptides of strain G-1, B. cinerea showed abnormal hyphal growth, deformed and swollen thalli, and leakage of internal contents. Analysis of differentially expressed genes indicated that 40 genes were differentially expressed at both time points. The gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that 15 genes related to terpene synthase and amino acid transmembrane transport were up-regulated, and 25 genes related to the cell surface receptor signaling pathway, oxidoreductase, pectin lyase and monooxygenase were down-regulated. Specifically, the expression levels of Bcin02g06840 gene encoding succinate dehydrogenase (SDH) and Bcin04g04450 gene b encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) were significantly down-regulated. The changes in the expression levels of eight differentially expressed genes according to qRT-PCR were consistent with the results of transcriptome sequencing analysis. These results indicated that the antimicrobial peptides of B. methylotrophicus G-1 might inhibit the growth of hyphae and spores by suppressing the synthesis of SDH and HMGR in the cell membrane components of B. cinerea.

Key words: Bacillus methylotrophicus, Botrytis cinerea, antimicrobial peptide, antifungal effect

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