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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (1): 258-266.DOI: 10.16409/j.cnki.2095-039x.2022.02.004

• RESEARCH REPORTS • Previous Articles     Next Articles

Identification of the Pathogen Causing Root Rot of Dendrobium officinale and Sensitivity to the Fermentation Broth of Streptomyces

ZHANG Xiangli, CAO Zhenyan, YANG Yihua, SONG Yang, SHENTU Xuping, YU Xiaoping   

  1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine/College of Life Science, China Jiliang University, Hangzhou 310018, China
  • Received:2020-08-11 Online:2022-02-08 Published:2022-03-01

Abstract: Root rot is one of the main diseases that have been harming Dendrobium officinale in Zhejiang Province. Pathogen identification is crucial to prevent and control root rot effectively. Fusarium sp. (No. GF-14) has been verified by Koch's rule, which is an important pathogen of D. officinale root rot. First, homology alignment and phylogenetic tree of sequences was analyzed, which included the internally transcribed spacer (rDNA-ITS), translation elongation factor (TEF-1α) and RNA polymerase genes (RPB1 and RPB2). At the same time, the morphological identification was also performed. It was confirmed that the pathogen GF-14 was F. incarnatum-equiseti species complex isolate. Afterwards, the susceptibility of GF-14 to a variety of fungicides was determined by mycelial growth rate method. The fungicide with best inhibitory effect on mycelial growth was selected to determine the field control effect. The results showed that the fermentation broth of Streptomyces diatatochromogenes 1628 diluted 10 times had the best biocontrol effect on the pathogenic fungus GF-14 (98.22%), while Ningnanmycin and Jinggangmycin had no effect on GF-14. Furthermore, the inhibitory rate of 1628 on the mycelial growth of GF-14 was 32.65% in the confrontation experiment, and the fermentation production of 1628 had a great biocontrol effect on root rot in the field (34.90%). This study clarified the pathogen classification of D. officinale root rot and proved the metabolites of 1628 could be used for biocontrol the disease in the future.

Key words: Dendrobium officinale, root rot, Fusarium, identification of pathogen, fermentation broth of Streptomyces diastatochramogenes

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