Molecular Mechanism of Azadirachtin Inhibiting Trehalase Activity Leading to Abnormal Molting and Metamorphosis of Helicoverpa armigera Larvae

doi:10.16409/j.cnki.2095-039x.2022.03.015

Abstract

Abstract: Azadirachtin is a botanical pesticide with good control effect on Lepidoptera, such as Helicoverpa armigera. In this study, the molecular mechanism of the death of H. armigera larvae after feeding with Azadirachtin was analyzed by the laboratory virulence assay, real-time quantitative PCR (qRT-PCR), and metabolite measurement. Firstly, the phenotype, body weight, mortality and pupation rate of the 5th and 6th instar larvae which were fed with different concentrations of Azadirachtin were analyzed. The results showed that Azadirachtin resulted in decreased body weight, increased mortality and decreased pupation rate of H. armigera larvae. Furthermore, we proved that Azadirachtin inhibiting the biosynthesis and hydrolysis of trehalose, the transcripts of 20E downstream genes, and reducing the ATP content through by qRT-PCR, enzyme activity and endogenous substance contents measurement. The results of this study indicate that Azadirachtin inhibited the biosynthesis and metabolism of trehalose, decreased the transcripts of 20E pathway genes, and reduced ATP production in H. armigera, so that causing molting and metamorphosis failure, and eventually led to the death of the larvae. This study is of great significance for further exploring the mechanism of Azadirachtin-induced death in H.armigera and control of pests by Azadirachtin.

Key words: Helicoverpa armigera, azadirachtin, trehalase, ATP, 20E pathway genes

CLC Number:

S476

CHANG Yanpeng, ZHANG Bo, GENG Zichen, WEI Jizhen, AN Shiheng, ZHAO Wenli. Molecular Mechanism of Azadirachtin Inhibiting Trehalase Activity Leading to Abnormal Molting and Metamorphosis of Helicoverpa armigera Larvae[J]. Chinese Journal of Biological Control, 2023, 39(3): 550-559.

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